Iman M Ahmad1, James B Temme1, Maher Y Abdalla2, Matthew C Zimmerman3. 1. a Radiation Science Technology Education, College of Allied Health Professions , University of Nebraska Medical Center , Omaha , USA. 2. b Department of Pathology and Microbiology , University of Nebraska Medical Center , Omaha , USA. 3. c Department of Cellular and Integrative Physiology , University of Nebraska Medical Center , Omaha , USA.
Abstract
OBJECTIVES: Reactive oxygen species (ROS), including superoxide (O2(•-)), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. METHODS: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2(•-) and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). RESULTS: Levels of O2(•-) and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. DISCUSSION: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.
OBJECTIVES:Reactive oxygen species (ROS), including superoxide (O2(•-)), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. METHODS: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2(•-) and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). RESULTS: Levels of O2(•-) and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. DISCUSSION: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.
Authors: Ali Akbar Malekirad; Akram Ranjbar; Kobra Rahzani; Ali Asghar Pilehvarian; Ali Rezaie; Mohammad Jafar Zamani; Mohammad Abdollahi Journal: Environ Toxicol Pharmacol Date: 2005-02-25 Impact factor: 4.860
Authors: Piotr Kłucinski; Aneta Wójcik; Rozalia Grabowska-Bochenek; Jan Gmiński; Bogdan Mazur; Antoni Hrycek; Paweł Cieślik; Gayane Martirosian Journal: Ann Agric Environ Med Date: 2008 Impact factor: 1.447
Authors: Jan M Zielinski; Michael J Garner; Pierre R Band; Daniel Krewski; Natalia S Shilnikova; Huixia Jiang; Patrick J Ashmore; Willem N Sont; Martha E Fair; Ernest G Letourneau; Robert Semenciw Journal: Int J Occup Med Environ Health Date: 2009 Impact factor: 1.843
Authors: V E Arterbery; W A Pryor; L Jiang; S S Sehnert; W M Foster; R A Abrams; J R Williams; M D Wharam; T H Risby Journal: Free Radic Biol Med Date: 1994-12 Impact factor: 7.376
Authors: Tiffany A Moore; Iman M Ahmad; Kendra K Schmid; Ann M Berger; R Jeanne Ruiz; Rita H Pickler; Matthew C Zimmerman Journal: Biol Res Nurs Date: 2019-07-08 Impact factor: 2.522
Authors: Rachel K Spooner; Brittany K Taylor; Iman M Ahmad; Kelsey N Dyball; Katy Emanuel; Howard S Fox; Kelly L Stauch; Matthew C Zimmerman; Tony W Wilson Journal: Proc Natl Acad Sci U S A Date: 2021-10-26 Impact factor: 11.205
Authors: Katalin Lumniczky; Nathalie Impens; Gemma Armengol; Serge Candéias; Alexandros G Georgakilas; Sabine Hornhardt; Olga A Martin; Franz Rödel; Dörthe Schaue Journal: Environ Int Date: 2020-12-05 Impact factor: 9.621