Literature DB >> 2674660

A new procedure for the targeted inactivation of essential bacterial genes.

T Lukacsovich1, P Venetianer.   

Abstract

A new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. The method is based on positive selection and is applicable for the exchange of essential genes. The main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong transcriptional termination signal into it; and (3) selection for double recombinants by means of the antibiotic resistance gene.

Mesh：

Year:  1989        PMID： 2674660     DOI： 10.1007/bf00331293

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  16 in total

1.  Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.

Authors:  R K Appleyard
Journal:  Genetics       Date:  1954-07       Impact factor: 4.562

2.  Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Authors:  S C Winans; S J Elledge; J H Krueger; G C Walker
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

3.  Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

Authors:  C M Joyce; N D Grindley
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

4.  Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication.

Authors:  L Covarrubias; F Bolivar
Journal:  Gene       Date:  1982-01       Impact factor: 3.688

5.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.

Authors:  J Brosius; T J Dull; D D Sleeter; H F Noller
Journal:  J Mol Biol       Date:  1981-05-15       Impact factor: 5.469

6.  Direct selection for the exchange of alleles between a plasmid and the Escherichia coli chromosome.

Authors:  A Merryweather; R Bernander; K Nordström
Journal:  Mol Gen Genet       Date:  1987-12

7.  Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.

Authors:  M Jasin; P Schimmel
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490

8.  Transposon Tn10 provides a promoter for transcription of adjacent sequences.

Authors:  M S Ciampi; M B Schmid; J R Roth
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205

9.  An Escherichia coli gene in search of a function.

Authors:  G Balikó; A Raukas; I Boros; P Venetianer
Journal:  Mol Gen Genet       Date:  1988-02

10.  Transcriptional termination at a fully rho-independent site in Escherichia coli is prevented by uninterrupted translation of the nascent RNA.

Authors:  J J Wright; R S Hayward
Journal:  EMBO J       Date:  1987-04       Impact factor: 11.598
View more
  1 in total

Review 1.  Chromosomal editing in Escherichia coli. Vectors for DNA integration and excision.

Authors:  P Balbás; G Gosset
Journal:  Mol Biotechnol       Date:  2001-09       Impact factor: 2.695
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.