Literature DB >> 2982787

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

S C Winans, S J Elledge, J H Krueger, G C Walker.   

Abstract

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

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Year:  1985        PMID： 2982787      PMCID： PMC215030          DOI： 10.1128/jb.161.3.1219-1221.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

1.  Genetic characterization of the metK locus in Escherichia coli K-12.

Authors:  J S Hunter; R C Greene; C H Su
Journal:  J Bacteriol       Date:  1975-06       Impact factor: 3.490

2.  The genetic and biochemical basis of the transformability of Escherichia coli K12.

Authors:  M Oishi; S D Cosloy
Journal:  Biochem Biophys Res Commun       Date:  1972-12-18       Impact factor: 3.575

3.  Amplification in Escherichia coli of enzymes involved in genetic recombination: construction of hybrid ColE1 plasmids carrying the structural gene for exonuclease I.

Authors:  D Vapnek; N K Alton; C L Bassett; S R Kushner
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

4.  Identification of pKM101-encoded loci specifying potentially lethal gene products.

Authors:  S C Winans; G C Walker
Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490

5.  Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.

Authors:  D J Garfinkel; R B Simpson; L W Ream; F F White; M P Gordon; E W Nester
Journal:  Cell       Date:  1981-11       Impact factor: 41.582

6.  Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions.

Authors:  J T Mulligan; W Margolin; J H Krueger; G C Walker
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

7.  Construction and characterization of a novel two-plasmid system for accomplishing temperature-regulated, amplified expression of cloned adventitious genes in Escherichia coli.

Authors:  J J Sninsky; B E Uhlin; P Gustafsson; S N Cohen
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

8.  Characterization of an endonuclease associated with the drug resistance plasmid pKM101.

Authors:  D Lackey; G C Walker; T Keng; S Linn
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

9.  Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.

Authors:  G B Ruvkun; V Sundaresan; F M Ausubel
Journal:  Cell       Date:  1982-06       Impact factor: 41.582

10.  ColE1 plasmid mutants affecting growth of an Escherichia coli recB recC sbcB mutant.

Authors:  J Inselburg
Journal:  J Bacteriol       Date:  1978-01       Impact factor: 3.490
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  189 in total

1.  Resistance to bacitracin as modulated by an Escherichia coli homologue of the bacitracin ABC transporter BcrC subunit from Bacillus licheniformis.

Authors:  Y M Harel; A Bailone; E Bibi
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  Competition between Escherichia coli strains expressing either a periplasmic or a membrane-bound nitrate reductase: does Nap confer a selective advantage during nitrate-limited growth?

Authors:  L C Potter; P Millington; L Griffiths; G H Thomas; J A Cole
Journal:  Biochem J       Date:  1999-11-15       Impact factor: 3.857

3.  The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription.

Authors:  J Dworkin; G Jovanovic; P Model
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

4.  Construction and characterization of mutations at codon 751 of the Escherichia coli gyrB gene that confer resistance to the antimicrobial peptide microcin B17 and alter the activity of DNA gyrase.

Authors:  F J del Castillo; I del Castillo; F Moreno
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

5.  Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose.

Authors:  E Ibañez; R Gimenez; T Pedraza; L Baldoma; J Aguilar; J Badia
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

6.  TonB interacts with nonreceptor proteins in the outer membrane of Escherichia coli.

Authors:  Penelope I Higgs; Tracy E Letain; Kelley K Merriam; Neal S Burke; HaJeung Park; ChulHee Kang; Kathleen Postle
Journal:  J Bacteriol       Date:  2002-03       Impact factor: 3.490

7.  TnphoA and TnphoA' elements for making and switching fusions for study of transcription, translation, and cell surface localization.

Authors:  M R Wilmes-Riesenberg; B L Wanner
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

8.  A protein methyltransferase specific for altered aspartyl residues is important in Escherichia coli stationary-phase survival and heat-shock resistance.

Authors:  C Li; S Clarke
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

9.  Flavodoxin mutants of Escherichia coli K-12.

Authors:  P Gaudu; B Weiss
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

10.  Characterization of the cryptic lambdoid prophage DLP12 of Escherichia coli and overlap of the DLP12 integrase gene with the tRNA gene argU.

Authors:  D F Lindsey; D A Mullin; J R Walker
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490
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