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Literature DB >> 2674182

The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis.

Abstract

The polymerase chain reaction (PCR) is a powerful method for the in vitro amplification of specific nucleic acid sequences. As very small amounts of a virus genome can be detected it has obvious diagnostic applications. The background to the reaction and its use for human immunodeficiency virus (HIV) detection are described. The problems likely to be encountered in using PCR as a diagnostic assay (false positives and negatives) and the practical measures which can be taken to overcome them are discussed.

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Year: 1989 PMID： 2674182 DOI： 10.1016/0166-0934(89)90031-1

Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014

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Cited

19 in total

1. Identification of a double-stranded RNA virus by using polymerase chain reaction and magnetic separation of the synthesized DNA segments.

Authors: E Rimstad; E Hornes; O Olsvik; B Hyllseth
Journal: J Clin Microbiol Date: 1990-10 Impact factor: 5.948

2. Post-PCR sterilization: development and application to an HIV-1 diagnostic assay.

Authors: S T Isaacs; J W Tessman; K C Metchette; J E Hearst; G D Cimino
Journal: Nucleic Acids Res Date: 1991-01-11 Impact factor: 16.971

Review 3. The polymerase chain reaction: current and future clinical applications.

Authors: J R Lynch; J M Brown
Journal: J Med Genet Date: 1990-01 Impact factor: 6.318

4. Fully automated detection of hepatitis C virus RNA in serum and whole-blood samples.

Authors: Harald H Kessler; Alexandra M K Clarici; Evelyn Stelzl; Gerhard Mühlbauer; Elisabeth Daghofer; Brigitte I Santner; Egon Marth; Rudolf E Stauber
Journal: Clin Diagn Lab Immunol Date: 2002-11

5. Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.

Authors: Kurt Beuselinck; Marc van Ranst; J van Eldere
Journal: J Clin Microbiol Date: 2005-11 Impact factor: 5.948

6. Comparison of in-house and commercial sample preparation and PCR amplification systems for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults.

Authors: E Lyamuya; U Bredberg-Rådén; J Albert; O Grankvist; V Msangi; C Kagoma; F Mhalu; G Biberfeld
Journal: J Clin Microbiol Date: 1997-01 Impact factor: 5.948

The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis.

1. Identification of a double-stranded RNA virus by using polymerase chain reaction and magnetic separation of the synthesized DNA segments.

2. Post-PCR sterilization: development and application to an HIV-1 diagnostic assay.

Review 3. The polymerase chain reaction: current and future clinical applications.

4. Fully automated detection of hepatitis C virus RNA in serum and whole-blood samples.

5. Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.

6. Comparison of in-house and commercial sample preparation and PCR amplification systems for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults.

7. Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid.

8. Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens.

9. Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera.

10. Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction.