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Literature DB >> 2668959

Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector.

H C Riethman¹, R K Moyzis, J Meyne, D T Burke, M V Olson.

Abstract

Telomeric fragments of human DNA ranging in size from 50 to 250 kilobases were cloned into Saccharomyces cerevisiae using a yeast-artificial-chromosome (YAC) vector. Six human-telomeric YAC (HTY) strains were selected by virtue of the specific hybridization of their DNA with the human telomeric terminal-repeat sequence (TTAGGG)n, and the telomeric localization of this sequence within each YAC was demonstrated by its sensitivity to nuclease BAL-31. In situ hybridization of DNA from three of these HTY strains with human metaphase chromosomes yielded discrete patterns of hybridization signals at the telomeres of a limited number of human chromosomes, different for each clone. DNA from selected cosmid subclones of one of the HTY strains was used to localize the origin of the cloned telomeric DNA by in situ hybridization to the tip of the long arm of chromosome 7.

Entities: Species

Mesh：

Substances：
DNA

Year: 1989 PMID： 2668959 PMCID： PMC297813 DOI： 10.1073/pnas.86.16.6240

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

43 in total

Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector.

1. A molecular description of telometic heterochromatin in secale species.

2. Isolation of a telomeric DNA sequence from Drosophila melanogaster.

3. Yeast centromeres: structure and function.

4. Construction of artificial chromosomes in yeast.

5. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

6. CDC17: an essential gene that prevents telomere elongation in yeast.

7. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena.

8. Cloning yeast telomeres on linear plasmid vectors.

9. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

10. Organization of DNA sequences and replication origins at yeast telomeres.

1. Size-dependent positioning of human chromosomes in interphase nuclei.

2. An integrated physical map for the short arm of human chromosome 5.

3. Mapping and initial analysis of human subtelomeric sequence assemblies.

Review 4. Minireview: cryptic translocations and telomere integrity.

5. Structural and transcriptional analysis of a human subtelomeric repeat.

6. Entire maize chloroplast genome is stably maintained in a yeast artificial chromosome.

7. Telomere-mediated chromosomal truncation in maize.

8. BAC clones generated from sheared DNA.

Review 9. The Thom Award address. Industrial mycology and the new genetics.

10. Highly conserved repetitive DNA sequences are present at human centromeres.