Zhi-Mei Xie1, Tao Zhou1, Hong-Yu Liao1, Qian Ye1, Shan Liu1, Lu Qi1, Jing Huang1, Hao-Jiang Zuo1, Xiao-Fang Pei1. 1. Zhi-Mei Xie, Tao Zhou, Hong-Yu Liao, Qian Ye, Shan Liu, Hao-Jiang Zuo, Xiao-Fang Pei, Department of Laboratory Science of Public Health, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan Province, China.
Abstract
AIM: To investigate the anti-obesity and antibacterial effects of Ligustrum robustum (L. robustum) in vivo and in vitro and its possible mechanisms. METHODS: The effects of L. robustum aqueous extract (LR) on various gut bacteria in vitro were evaluated. The effects of LR on high-fat diet-fed (HFD) rats in vivo were also assessed. Culture methods, quantitative polymerase chain reaction, and terminal-restriction fragment length polymorphism were used to analyze the effects of LR on gut bacteria. Biochemical tests were also performed to detect the changes in obesity-related indicators after LR treatment. RESULTS: LR treatment lowered adipose weight and decreased Lee's index, blood glucose, total cholesterol, and lipid in the tested groups relative to control (P < 0.05). To determine the reasons for these changes, we assessed the potential bacteriostatic and bactericidal effects of LR on specific bacterial species in vitro. LR affected the richness, diversity, and evenness of gut bacteria, increased fecal Lactobacillus, and decreased Enterococci in HFD rats (P < 0.05). CONCLUSION: L. robustum may be a safe and effective food for weight loss and obesity control, and the effects of L. robustum might be mediated by the regulation of gut bacteria.
AIM: To investigate the anti-obesity and antibacterial effects of Ligustrum robustum (L. robustum) in vivo and in vitro and its possible mechanisms. METHODS: The effects of L. robustum aqueous extract (LR) on various gut bacteria in vitro were evaluated. The effects of LR on high-fat diet-fed (HFD) rats in vivo were also assessed. Culture methods, quantitative polymerase chain reaction, and terminal-restriction fragment length polymorphism were used to analyze the effects of LR on gut bacteria. Biochemical tests were also performed to detect the changes in obesity-related indicators after LR treatment. RESULTS: LR treatment lowered adipose weight and decreased Lee's index, blood glucose, total cholesterol, and lipid in the tested groups relative to control (P < 0.05). To determine the reasons for these changes, we assessed the potential bacteriostatic and bactericidal effects of LR on specific bacterial species in vitro. LR affected the richness, diversity, and evenness of gut bacteria, increased fecal Lactobacillus, and decreased Enterococci in HFD rats (P < 0.05). CONCLUSION:L. robustum may be a safe and effective food for weight loss and obesity control, and the effects of L. robustum might be mediated by the regulation of gut bacteria.
Entities:
Keywords:
Culture methods; Gut bacteria; Ligustrum robustum; Obesity; Quantitative polymerase chain reaction; Terminal-restriction fragment length polymorphism
Authors: Barbara U Metzler-Zebeli; Seema Hooda; Robert Pieper; Ruurd T Zijlstra; Andrew G van Kessel; Rainer Mosenthin; Michael G Gänzle Journal: Appl Environ Microbiol Date: 2010-04-09 Impact factor: 4.792
Authors: I Nadal; A Santacruz; A Marcos; J Warnberg; J M Garagorri; M Garagorri; L A Moreno; M Martin-Matillas; C Campoy; A Martí; A Moleres; M Delgado; O L Veiga; M García-Fuentes; C G Redondo; Y Sanz Journal: Int J Obes (Lond) Date: 2008-12-09 Impact factor: 5.095