Literature DB >> 26640761

Assessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure.

Gregory W Watson1, Samanthi Wickramasekara2, Claudia S Maier2, David E Williams3, Roderick H Dashwood4, Emily Ho5.   

Abstract

The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

Entities:  

Keywords:  mass spectrometry; prostate cancer; sulforaphane

Year:  2015        PMID： 26640761      PMCID： PMC4666544          DOI： 10.1016/j.euprot.2015.08.002

Source DB:  PubMed          Journal:  EuPA Open Proteom        ISSN： 2212-9685


  36 in total

1.  Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.

Authors:  Andrew Keller; Alexey I Nesvizhskii; Eugene Kolker; Ruedi Aebersold
Journal:  Anal Chem       Date:  2002-10-15       Impact factor: 6.986

2.  Evaluation of comprehensive multidimensional separations using reversed-phase, reversed-phase liquid chromatography/mass spectrometry for shotgun proteomics.

Authors:  Tatsuji Nakamura; Junro Kuromitsu; Yoshiya Oda
Journal:  J Proteome Res       Date:  2008-02-02       Impact factor: 4.466

3.  Comparative analysis of OFFGel, strong cation exchange with pH gradient, and RP at high pH for first-dimensional separation of peptides from a membrane-enriched protein fraction.

Authors:  Bruno Manadas; Jane A English; Kieran J Wynne; David R Cotter; Michael J Dunn
Journal:  Proteomics       Date:  2009-11       Impact factor: 3.984

Review 4.  Multi-dimensional liquid chromatography in proteomics--a review.

Authors:  Xiang Zhang; Aiqin Fang; Catherine P Riley; Mu Wang; Fred E Regnier; Charles Buck
Journal:  Anal Chim Acta       Date:  2010-02-06       Impact factor: 6.558

5.  Integrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression.

Authors:  Sooryanarayana Varambally; Jianjun Yu; Bharathi Laxman; Daniel R Rhodes; Rohit Mehra; Scott A Tomlins; Rajal B Shah; Uma Chandran; Federico A Monzon; Michael J Becich; John T Wei; Kenneth J Pienta; Debashis Ghosh; Mark A Rubin; Arul M Chinnaiyan
Journal:  Cancer Cell       Date:  2005-11       Impact factor: 31.743

6.  p53CSV, a novel p53-inducible gene involved in the p53-dependent cell-survival pathway.

Authors:  Woong-Ryeon Park; Yusuke Nakamura
Journal:  Cancer Res       Date:  2005-02-15       Impact factor: 12.701

Review 7.  Molecular basis for chemoprevention by sulforaphane: a comprehensive review.

Authors:  N Juge; R F Mithen; M Traka
Journal:  Cell Mol Life Sci       Date:  2007-05       Impact factor: 9.261

8.  Sulforaphane inhibits prostate carcinogenesis and pulmonary metastasis in TRAMP mice in association with increased cytotoxicity of natural killer cells.

Authors:  Shivendra V Singh; Renaud Warin; Dong Xiao; Anna A Powolny; Silvia D Stan; Julie A Arlotti; Yan Zeng; Eun-Ryeong Hahm; Stanley W Marynowski; Ajay Bommareddy; Dhimant Desai; Shantu Amin; Robert A Parise; Jan H Beumer; William H Chambers
Journal:  Cancer Res       Date:  2009-02-17       Impact factor: 12.701

9.  Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells.

Authors:  Chan-Hee Lee; Soo-Jin Jeong; Sun-Mi Yun; Ji-Hyun Kim; Hyo-Jung Lee; Kwang Seok Ahn; Suk-Hyun Won; Hyun Seok Kim; Hyo-Jeong Lee; Kyoo-Seok Ahn; Shudong Zhu; Chang-Yan Chen; Sung-Hoon Kim
Journal:  Proteome Sci       Date:  2010-12-16       Impact factor: 2.480

Review 10.  Molecular mechanisms of translational control.

Authors:  Fátima Gebauer; Matthias W Hentze
Journal:  Nat Rev Mol Cell Biol       Date:  2004-10       Impact factor: 94.444
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