Fengbo Zhang1, Zhiwei Li2, Xiaolin La3, Xiumin Ma4, Yaoxin Zhang5, Ping Ji4, Min Jiang6, Jinwei Hu4, Zhaoxia Zhang4, Xiaobo Lu5, Jianbing Ding1. 1. Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China ; Department of Immunology, School of Preclinical Medicine, Xinjiang Medical University Urumqi 830011, Xinjiang, China. 2. Department of Clinical Laboratory, Xinjiang People's Hospital Urumqi 830000, Xinjiang, China. 3. Reproductive Medicine Center, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China. 4. Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China. 5. Department of Infectious Diseases, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China. 6. Department of Immunology, School of Preclinical Medicine, Xinjiang Medical University Urumqi 830011, Xinjiang, China.
Abstract
OBJECTIVE: This study is to characterize and identify the human Brucella strains in Xinjiang, China with multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme. METHODS: Brucella strains were isolated and cultured from 62 brucellosis patients. The bacteria strains were subjected to the oxidase, catalase, rapid urease, and nitrate reduction tests, and the species identification was performed using the VITEK-2 Compact system. These Brucella strains were further identified and characterized using the 16 VNTR loci in a MLVA-16 methodology. RESULTS: Twelve Brucella strains had been identified out of 62 patients, which were all recognized as Brucella melitensis (B. melitensis) according to the results from the VITEK-2 Compact system. Based on panel 1 (MLVA-8), these 12 Brucella isolates were clustered into three known genotypes and two new genotypes, in which 7 strains were clustered into genotype 45 (1-5-3-12-2-2-3-2), 1 strain was classified as genotype 42 (1-5-3-13-2-2-3-2), 1 stain was with genotype 62 (1-3-3-13-2-2-3-2), and the other 3 trains revealed two new genotypes, i.e., (1-5-3-12-2-3-3-2) and (1-5-3-11-2-3-3-2). Using panel 2A+2B (MLVA-16), we found that no genotypes of these strains were identical to the known genotypes, generally with differences in 2-4 loci. However, three strains shared the same genotype. CONCLUSION: Brucella strains in 62 brucellosis patients from Xinjiang are all identified as B. melitensis. Based on MLVA-8, two new genotypes have been discovered. These findings might contribute to the understanding of the pathogenesis and epidemiology of brucellosis in Xinjiang, China.
OBJECTIVE: This study is to characterize and identify the human Brucella strains in Xinjiang, China with multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme. METHODS: Brucella strains were isolated and cultured from 62 brucellosispatients. The bacteria strains were subjected to the oxidase, catalase, rapid urease, and nitrate reduction tests, and the species identification was performed using the VITEK-2 Compact system. These Brucella strains were further identified and characterized using the 16 VNTR loci in a MLVA-16 methodology. RESULTS: Twelve Brucella strains had been identified out of 62 patients, which were all recognized as Brucella melitensis (B. melitensis) according to the results from the VITEK-2 Compact system. Based on panel 1 (MLVA-8), these 12 Brucella isolates were clustered into three known genotypes and two new genotypes, in which 7 strains were clustered into genotype 45 (1-5-3-12-2-2-3-2), 1 strain was classified as genotype 42 (1-5-3-13-2-2-3-2), 1 stain was with genotype 62 (1-3-3-13-2-2-3-2), and the other 3 trains revealed two new genotypes, i.e., (1-5-3-12-2-3-3-2) and (1-5-3-11-2-3-3-2). Using panel 2A+2B (MLVA-16), we found that no genotypes of these strains were identical to the known genotypes, generally with differences in 2-4 loci. However, three strains shared the same genotype. CONCLUSION: Brucella strains in 62 brucellosispatients from Xinjiang are all identified as B. melitensis. Based on MLVA-8, two new genotypes have been discovered. These findings might contribute to the understanding of the pathogenesis and epidemiology of brucellosis in Xinjiang, China.
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