Qing Chen1,2, Kshitij Srivastava3, Zhong Liu4, Jianyu Xiao1, Chengyin Huang1, Jun Sun1, Min Li5,6, Willy Albert Flegel3. 1. Jiangsu Province Blood Center, Nanjing. 2. Soochow University, Suzhou, Jiangsu, China. 3. Department of Transfusion Medicine, NIH Clinical Center, National Institutes of Health, Bethesda, Maryland. 4. Institute of Blood Transfusion, Chinese Academy of Medical Science & Peking Union Medical College, Chengdu, Sichuan, China. 5. Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College. 6. Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, Jiangsu, China.
Abstract
BACKGROUND: Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by polymerase chain reaction with sequence-specific priming (PCR-SSP). We sequenced SLC44A2 gene fragments and determined allele frequencies in three ethnicities of China. STUDY DESIGN AND METHODS: Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of two single-nucleotide polymorphisms (SNPs); SLC44A2 haplotypes were constructed. RESULTS: In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66, and 0.69 in Yi, Han (Nanjing), and Tibetan, respectively (0.42, 0.34, and 0.31 for HNA-3b). CONCLUSIONS: The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations.
BACKGROUND:Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by polymerase chain reaction with sequence-specific priming (PCR-SSP). We sequenced SLC44A2 gene fragments and determined allele frequencies in three ethnicities of China. STUDY DESIGN AND METHODS: Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of two single-nucleotide polymorphisms (SNPs); SLC44A2 haplotypes were constructed. RESULTS: In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66, and 0.69 in Yi, Han (Nanjing), and Tibetan, respectively (0.42, 0.34, and 0.31 for HNA-3b). CONCLUSIONS: The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations.
Authors: M de Haas; E Muniz-Diaz; L G Alonso; K van der Kolk; M Kos; L Buddelmeijer; L Porcelijn; A E von dem Borne Journal: Transfusion Date: 2000-02 Impact factor: 3.157
Authors: A Davoren; B R Curtis; I A Shulman; A F Mohrbacher; J Bux; B J Kwiatkowska; J G McFarland; R H Aster Journal: Transfusion Date: 2003-05 Impact factor: 3.157
Authors: Christopher C Silliman; Brian R Curtis; Patricia M Kopko; Samina Y Khan; Marguerite R Kelher; Randy M Schuller; Baindu Sannoh; Daniel R Ambruso Journal: Blood Date: 2006-10-12 Impact factor: 22.113
Authors: J Y Chu; W Huang; S Q Kuang; J M Wang; J J Xu; Z T Chu; Z Q Yang; K Q Lin; P Li; M Wu; Z C Geng; C C Tan; R F Du; L Jin Journal: Proc Natl Acad Sci U S A Date: 1998-09-29 Impact factor: 11.205