Literature DB >> 26567472

Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.

Todd A Duncombe1, Chi-Chih Kang1, Santanu Maity1, Toby M Ward2, Mark D Pegram2, Niren Murthy1, Amy E Herr1.   

Abstract

Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  cancer therapies; gradient gels; grayscale masks; hydrogel pore expansion; western blots

Mesh：

Substances：

Year:  2015        PMID： 26567472      PMCID： PMC4708057          DOI： 10.1002/adma.201503939

Source DB:  PubMed          Journal:  Adv Mater        ISSN： 0935-9648            Impact factor:   30.849


  42 in total

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Authors:  Dietmar W Hutmacher
Journal:  Nat Mater       Date:  2010-02       Impact factor: 43.841

2.  Isolation of intact proteins from acid-degradable polyacrylamide gel.

Authors:  Yoon Kyung Kim; Young Jik Kwon
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3.  Extracellular matrix stiffness and composition jointly regulate the induction of malignant phenotypes in mammary epithelium.

Authors:  Ovijit Chaudhuri; Sandeep T Koshy; Cristiana Branco da Cunha; Jae-Won Shin; Catia S Verbeke; Kimberly H Allison; David J Mooney
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4.  Polyacrylamide gel-electrophoresis across a molecular sieve gradient.

Authors:  J Margolis; K C Kenrick
Journal:  Nature       Date:  1967-06-24       Impact factor: 49.962

Review 5.  Optimal targeting of HER2-PI3K signaling in breast cancer: mechanistic insights and clinical implications.

Authors:  Brent N Rexer; Carlos L Arteaga
Journal:  Cancer Res       Date:  2013-06-21       Impact factor: 12.701

6.  Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene.

Authors:  D J Slamon; G M Clark; S G Wong; W J Levin; A Ullrich; W L McGuire
Journal:  Science       Date:  1987-01-09       Impact factor: 47.728

7.  Western blotting using microchip electrophoresis interfaced to a protein capture membrane.

Authors:  Shi Jin; Gwendolyn J Anderson; Robert T Kennedy
Journal:  Anal Chem       Date:  2013-05-28       Impact factor: 6.986

Review 8.  Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis.

Authors:  Edward C Stack; Chichung Wang; Kristin A Roman; Clifford C Hoyt
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9.  Controlling uniformity of photopolymerized microscopic hydrogels.

Authors:  Sukho Park; Dongshin Kim; Seong Young Ko; Jong-Oh Park; Sathish Akella; Bing Xu; Ye Zhang; Seth Fraden
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Review 10.  Hallmarks of cancer: the next generation.

Authors:  Douglas Hanahan; Robert A Weinberg
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  25 in total

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Review 3.  Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.

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4.  Ferguson analysis of protein electromigration during single-cell electrophoresis in an open microfluidic device.

Authors:  Kristine Y Tan; Amy E Herr
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Review 5.  Recent advances in protein analysis by capillary and microchip electrophoresis.

Authors:  Mohamed Dawod; Natalie E Arvin; Robert T Kennedy
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Review 6.  Recent Advances in the Analysis of Single Cells.

Authors:  Lucas Armbrecht; Petra S Dittrich
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7.  Detection of Isoforms Differing by a Single Charge Unit in Individual Cells.

Authors:  Augusto M Tentori; Kevin A Yamauchi; Amy E Herr
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8.  Kinetic Analysis of Enzymes Immobilized in Porous Film Arrays.

Authors:  Hector D Neira; Amy E Herr
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9.  Single cell-resolution western blotting.

Authors:  Chi-Chih Kang; Kevin A Yamauchi; Julea Vlassakis; Elly Sinkala; Todd A Duncombe; Amy E Herr
Journal:  Nat Protoc       Date:  2016-07-28       Impact factor: 13.491

Review 10.  Microchip-based single-cell functional proteomics for biomedical applications.

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Journal:  Lab Chip       Date:  2017-03-29       Impact factor: 6.799
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