Literature DB >> 265579

Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.

P Bhattacharya, J R Moskal, S Basu.   

Abstract

Activity of a trypsin-sensitive acid deoxyribonuclease (DNase) inhibitor has been detected in 13- to 21-day-old embryonic chicken brains and in clonal lines of neuroblastoma cells (adrenergic, N1E-115, and neurotransmitter-inactive, N-18) grown for 48 hr after subculture. The activities of purified porcine and bovine spleen DNases were inhibited 60--75% in the presence of the inhibitor, whereas less than 10% inhibition was observed with purified pancreatic DNase. Activities of an acidic DNase and its inhibitor reached maxima in 21-day-old embryonic chicken brain. The proteins were separated by isoelectric focusing and affinity column chromatographic techniques. The pI values of the acid DNase and its inhibitor were 5.4 and 4.2, respectively.

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Year:  1977        PMID： 265579      PMCID： PMC430497          DOI： 10.1073/pnas.74.3.842

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Nonhistone chromosomal proteins and gene regulation.

Authors:  G S Stein; T C Spelsberg; L J Kleinsmith
Journal:  Science       Date:  1974-03-01       Impact factor: 47.728

4.  Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells.

Authors:  J R Moskal; D A Gardner; S Basu
Journal:  Biochem Biophys Res Commun       Date:  1974-11-27       Impact factor: 3.575

5.  Morphologic differentiation of mouse neuroblastoma cells induced in vitro by dibutyryl adenosine 3':5'-cyclic monophosphate.

Authors:  K N Prasad; A W Hsie
Journal:  Nat New Biol       Date:  1971-09-29

6.  Some aspects of the desoxyribonuclease activities of animal tissues.

Authors:  V ALLFREY; A E MIRSKY
Journal:  J Gen Physiol       Date:  1952-11       Impact factor: 4.086

7.  Regulation of tyrosine hydroxylase activity in cultured mouse neuroblastoma cells: elevation induced by analogs of adenosine 3':5'-cyclic monophosphate.

Authors:  J C Waymire; N Weiner; K N Prasad
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites).

Authors:  T Amano; E Richelson; M Nirenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205

9.  Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.

Authors:  J Minna; P Nelson; J Peacock; D Glazer; M Nirenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1971-01       Impact factor: 11.205

10.  Actin is the naturally occurring inhibitor of deoxyribonuclease I.

Authors:  E Lazarides; U Lindberg
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205
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  5 in total

1.  DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.

Authors:  P Bhattacharya; S Basu
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

2.  Inhibition of human neuroblastoma DNA polymerase activities by plant lectins and toxins.

Authors:  P Bhattacharya; I Simet; S Basu
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

3.  Differential inhibition of multiple forms of DNA polymerase alpha from IMR-32 human neuroblastoma cells.

Authors:  P Bhattacharya; I Simet; S Basu
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

4.  Stimulation of human neuroblastoma DNA polymerase alpha and primase activities by a protein factor isolated from rat liver chromatin.

Authors:  S Takada; A Torres-Rosado; S Ray; S Basu
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

5.  [DNase II activation in rat liver in the course of acute diethylnitrosamine-induced damage (author's transl)].

Authors:  I Schmerold; K Tempel
Journal:  Arch Toxicol       Date:  1981-09       Impact factor: 5.153
  5 in total

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