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Literature DB >> 274718

DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.

Abstract

The activities of two DNA polymerases (DNA nucleotidyltransferases) were characterized in mouse neuroblastoma clone N-18 on the basis of their apparent molecular weights (determined by sucrose density gradient centrifugation: polymerase-alpha, 7.5-8 S; polymerase-beta, 3-4 S) and relative inhibition by sulfhydryl-blocking agents. N-Ethylmaleimide (10 mM) and iodoacetamide (1.5 mM) inhibited DNA polymerase-alpha activity completely, whereas only 35-40% inhibition was observed for DNA polymerase-beta under similar conditions. DNA polymerase-alpha activity was reduced 50-70% in N-18 cells that had been induced to differentiate by 4 micro M bromodeoxyuridine, and the low molecular weight DNA polymerase-beta activity remain unchanged. With activated calf thymus DNA as template, only DNA polymerase-alpha activity was stimulated in the presence of added ribonucleotides and purified Escherichia coli RNA polymerase.

Entities: Chemical Disease Gene Species

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Year: 1978 PMID： 274718 PMCID： PMC411456 DOI： 10.1073/pnas.75.3.1289

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

48 in total

1. Nuclear DNA-dependent DNA polymerases in human brain.

Authors: M V Viola; P Norton; M L Cole
Journal: J Neurochem Date: 1977-01 Impact factor: 5.372

2. DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES.

Authors: M YONEDA; F J BOLLUM
Journal: J Biol Chem Date: 1965-08 Impact factor: 5.157

3. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

Authors: H V APOSHIAN; A KORNBERG
Journal: J Biol Chem Date: 1962-02 Impact factor: 5.157

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.

Authors: P Bhattacharya; J R Moskal; S Basu
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

Review 6. Eukaryotic DNA polymerases.

Authors: A Weissbach
Journal: Annu Rev Biochem Date: 1977 Impact factor: 23.643

DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.

1. Nuclear DNA-dependent DNA polymerases in human brain.

2. DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES.

3. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

4. Protein measurement with the Folin phenol reagent.

5. Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.

Review 6. Eukaryotic DNA polymerases.

7. Enzymatic initiation of DNA synthesis by yeast DNA polymerases.

8. Determination of 5'-bromodeoxyuridine in DNA by buoyant density.

9. Removal of 5-bromo-2-deoxyuridine incorporated in DNA of regenerating rat liver.

10. Changes in type of collagen synthesized by chick fibroblasts in vitro in the presence of 5-bromodeoxyuridine.

1. Inhibition of human neuroblastoma DNA polymerase activities by plant lectins and toxins.

2. Differential inhibition of multiple forms of DNA polymerase alpha from IMR-32 human neuroblastoma cells.