Veria Khosrawipour1,2, Tanja Khosrawipour2, David Diaz-Carballo3, Eckart Förster4, Jürgen Zieren1,2, Urs Giger-Pabst5,6. 1. Department of General Surgery & Therapy Center for Peritonealcarcinomatosis, St. Mary's Hospital Herne, Ruhr University Bochum, Herne, Germany. 2. Basic Research Laboratory Department of Surgery, St. Mary's Hospital Herne, Ruhr University Bochum, Herne, Germany. 3. Department of Hematology & Medical Oncology, St. Mary's Hospital Herne, Ruhr University Bochum, Herne, Germany. 4. Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Herne, Germany. 5. Department of General Surgery & Therapy Center for Peritonealcarcinomatosis, St. Mary's Hospital Herne, Ruhr University Bochum, Herne, Germany. urs.pabst@marienhospital-herne.de. 6. Basic Research Laboratory Department of Surgery, St. Mary's Hospital Herne, Ruhr University Bochum, Herne, Germany. urs.pabst@marienhospital-herne.de.
Abstract
BACKGROUND: Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a novel approach to delivering intraperitoneal chemotherapy (IPC) as a pressurized aerosol. One of the assumed advantages is the homogeneous drug distribution in the intraperitoneal cavity compared with conventional liquid in situ chemotherapy. However, to date, the spatial drug distribution pattern of PIPAC has not been investigated in detail. METHODS: Doxorubicin was aerosolized in an ex vivo PIPAC model containing native fresh tissue samples of swine peritoneum at a pressure of 12 mmHg CO2 at 36 °C. In the center of the top cover of the PIPAC chamber, a PIPAC micropump was installed. Tissue specimens were placed as follows: (A) bottom of the plastic box, (B) margin of the aerosol jet covered with a bilaterally open tunnel, (C) side wall, and (D) top cover, respectively. In-tissue doxorubicin penetration was measured using fluorescence microscopy on frozen thin sections. RESULTS: The depth of doxorubicin penetration was found to be significantly higher in tissues directly exposed to the aerosol jet (A: 215 ± 79 µm) compared with the side wall (C: 77 ± 18 µm; p < 0.01) and the top of the box (D: 65 ± 17 µm; p < 0.01). The poorest penetration was observed for peritoneal tissue covered under a bilaterally open plastic tunnel (B: 34 ± 19 µm; p < 0.001). CONCLUSIONS: The study data suggest that the spatial drug distribution pattern of ex vivo PIPAC is heterogeneous.
BACKGROUND: Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a novel approach to delivering intraperitoneal chemotherapy (IPC) as a pressurized aerosol. One of the assumed advantages is the homogeneous drug distribution in the intraperitoneal cavity compared with conventional liquid in situ chemotherapy. However, to date, the spatial drug distribution pattern of PIPAC has not been investigated in detail. METHODS:Doxorubicin was aerosolized in an ex vivo PIPAC model containing native fresh tissue samples of swine peritoneum at a pressure of 12 mmHg CO2 at 36 °C. In the center of the top cover of the PIPAC chamber, a PIPAC micropump was installed. Tissue specimens were placed as follows: (A) bottom of the plastic box, (B) margin of the aerosol jet covered with a bilaterally open tunnel, (C) side wall, and (D) top cover, respectively. In-tissue doxorubicin penetration was measured using fluorescence microscopy on frozen thin sections. RESULTS: The depth of doxorubicin penetration was found to be significantly higher in tissues directly exposed to the aerosol jet (A: 215 ± 79 µm) compared with the side wall (C: 77 ± 18 µm; p < 0.01) and the top of the box (D: 65 ± 17 µm; p < 0.01). The poorest penetration was observed for peritoneal tissue covered under a bilaterally open plastic tunnel (B: 34 ± 19 µm; p < 0.001). CONCLUSIONS: The study data suggest that the spatial drug distribution pattern of ex vivo PIPAC is heterogeneous.
Authors: Rafael Seitenfus; Antonio Nocchi Kalil; Eduardo Dipp de Barros; Claudio Galeano Zettler; Gabriel Oliveira Dos Santos; Olivier Glehen; Carlos Humberto Cereser Junior; Paulo Roberto Walter Ferreira Journal: Surg Endosc Date: 2019-08-01 Impact factor: 4.584
Authors: Daniel Göhler; Veria Khosrawipour; Tanja Khosrawipour; David Diaz-Carballo; Thomas Albert Falkenstein; Jürgen Zieren; Michael Stintz; Urs Giger-Pabst Journal: Surg Endosc Date: 2016-09-08 Impact factor: 4.584
Authors: Alexander Bellendorf; Veria Khosrawipour; Tanja Khosrawipour; Simon Siebigteroth; Joseph Cohnen; David Diaz-Carballo; Andreas Bockisch; Jürgen Zieren; Urs Giger-Pabst Journal: Surg Endosc Date: 2017-06-22 Impact factor: 4.584