Tao Li1, Yuhua Zhu1, Lihui Han2, Wanhua Ren1, Hui Liu1, Chengyong Qin1. 1. Department of Infectious diseases, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. 2. Department of Immunology, Shandong University School of Medicine, Jinan 250012, China.
Abstract
AIM: VEGFR-1 can promote invasion through epithelial-mesenchymal transition induction in hepatocellular carcinoma (HCC). This study aims to elucidate VEGFR-1 impact on proteolytic enzymes profile involved with invasion. MATERIALS & METHODS: The effect on cell invasion was evaluated by invasive and migration assays with and without VEGFR-1 activation. The mechanism was investigated by real-time PCR, western blot and gelatin zymography using inhibitors for MMP-9. In total, 95 HCC patients were enrolled for its clinical value evaluation. RESULTS: VEGFR-1 activation induced invasion in HCC cells with an increase in the expression and activity of MMP-9 and Snail. MMP-9 blockage effectively inhibited VEGFR-1-induced invasion. High coexpression of both in HCC predicted a worse clinical outcome. CONCLUSION: Data show a novel VEGFR-1 activation-to-MMP-9 mechanism promoting HCC invasion.
AIM: VEGFR-1 can promote invasion through epithelial-mesenchymal transition induction in hepatocellular carcinoma (HCC). This study aims to elucidate VEGFR-1 impact on proteolytic enzymes profile involved with invasion. MATERIALS & METHODS: The effect on cell invasion was evaluated by invasive and migration assays with and without VEGFR-1 activation. The mechanism was investigated by real-time PCR, western blot and gelatin zymography using inhibitors for MMP-9. In total, 95 HCC patients were enrolled for its clinical value evaluation. RESULTS:VEGFR-1 activation induced invasion in HCC cells with an increase in the expression and activity of MMP-9 and Snail. MMP-9 blockage effectively inhibited VEGFR-1-induced invasion. High coexpression of both in HCC predicted a worse clinical outcome. CONCLUSION: Data show a novel VEGFR-1 activation-to-MMP-9 mechanism promoting HCC invasion.
Authors: Alexander Laird; Fiach C O'Mahony; Jyoti Nanda; Antony C P Riddick; Marie O'Donnell; David J Harrison; Grant D Stewart Journal: PLoS One Date: 2013-04-05 Impact factor: 3.240
Authors: Heba M I Abdallah; Sally A El Awdan; Rehab F Abdel-Rahman; Abdel Razik H Farrag; Rasha M Allam Journal: PLoS One Date: 2022-01-26 Impact factor: 3.240