| Literature DB >> 21140254 |
Jianhua Tang1, Jiefeng Cui, Rongxin Chen, Kun Guo, Xiaonan Kang, Yan Li, Dongmei Gao, Lu Sun, Changde Xu, Jie Chen, Zhaoyou Tang, Yinkun Liu.
Abstract
We established an in vitro 3-D model of metastatic hepatocellular carcinoma (HCC) by culturing MHCC97H cells on molecular scaffolds within a rotating wall vessel bioreactor. Morphological and biochemical analyses revealed that the 3-D HCC model mirrored many clinical pathological features of HCC in vivo, including cancer cell morphology, tissue ultrastructure, protein production and secretion, glucose metabolism, tissue-specific gene expression, and apoptosis. Xenografts into livers of nude mice resulted in tumorigenesis and distant metastasis. This 3-D HCC spheroid is a promising model for HCC tumor biology, anticancer drug screening, and for the establishment of HCC animal models.Entities:
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Year: 2010 PMID： 21140254 DOI： 10.1007/s13277-010-0140-7
Source DB: PubMed Journal: Tumour Biol ISSN： 1010-4283