E Kerambrun1, M Palos Ladeiro1,2, A Bigot-Clivot1, O Dedourge-Geffard1, E Dupuis2, I Villena2, D Aubert2, A Geffard1. 1. Unité Stress Environnementaux et Biosurveillance des milieux aquatiques, UMR-I 02 (SEBIO), Université de Reims Champagne Ardenne, Reims, France. 2. Laboratoire de Parasitologie-Mycologie, EA 3800 SFR CAP-Santé FED 4231, Hôpital Maison Blanche, Reims, France.
Abstract
AIMS: The objective of this study was to evaluate if freshwater bivalves can be used to detect the presence of Toxoplasma gondii in water bodies. METHODS AND RESULTS: Zebra mussels (Dreissena polymorpha) were caged for 1 month upstream and downstream of the discharge points of wastewater treatment plants (WWTPs). Physiological status was assessed to assure good health of bivalves during transplantation. The presence of T. gondii was investigated in mussel tissues by qPCR. In autumn, T. gondii was detected in mussels caged downstream of the discharge points of two WWTPs. In spring, it was detected upstream of one WWTP. CONCLUSIONS: For the first time, T. gondii DNA has been shown in a continental mollusc in environmental conditions. This highlights the interest of an active approach that could be applied independently of the presence or accessibility of autochthonous populations, and underlines the presence of T. gondii in natural waters under pressure of WWTP discharge at a certain time of the year. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows that transplanted zebra mussels could be used as biosamplers to reveal contamination of freshwater systems by T. gondii.
AIMS: The objective of this study was to evaluate if freshwater bivalves can be used to detect the presence of Toxoplasma gondii in water bodies. METHODS AND RESULTS:Zebra mussels (Dreissena polymorpha) were caged for 1 month upstream and downstream of the discharge points of wastewater treatment plants (WWTPs). Physiological status was assessed to assure good health of bivalves during transplantation. The presence of T. gondii was investigated in mussel tissues by qPCR. In autumn, T. gondii was detected in mussels caged downstream of the discharge points of two WWTPs. In spring, it was detected upstream of one WWTP. CONCLUSIONS: For the first time, T. gondii DNA has been shown in a continental mollusc in environmental conditions. This highlights the interest of an active approach that could be applied independently of the presence or accessibility of autochthonous populations, and underlines the presence of T. gondii in natural waters under pressure of WWTP discharge at a certain time of the year. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows that transplanted zebra mussels could be used as biosamplers to reveal contamination of freshwater systems by T. gondii.
Authors: Ana Tereza Galvani; Ana Paula Guarnieri Christ; José Antonio Padula; Mikaela Renata Funada Barbosa; Ronalda Silva de Araújo; Maria Inês Zanoli Sato; Maria Tereza Pepe Razzolini Journal: Parasitol Res Date: 2019-01-04 Impact factor: 2.289
Authors: Alexandra Zieritz; Ronaldo Sousa; David C Aldridge; Karel Douda; Eduardo Esteves; Noé Ferreira-Rodríguez; Jon H Mageroy; Daniele Nizzoli; Martin Osterling; Joaquim Reis; Nicoletta Riccardi; Daniel Daill; Clemens Gumpinger; Ana Sofia Vaz Journal: Biol Rev Camb Philos Soc Date: 2022-06-30
Authors: Nadia María López Ureña; Umer Chaudhry; Rafael Calero Bernal; Santiago Cano Alsua; Davide Messina; Francisco Evangelista; Martha Betson; Marco Lalle; Pikka Jokelainen; Luis Miguel Ortega Mora; Gema Álvarez García Journal: Microorganisms Date: 2022-02-27