Ayman Hammad1, Youssef M Mosaad2, Enas M Hammad3, Samir Elhanbly4, Sherif R El-Bassiony3, Mohammad F Al-Harrass2, Riham Eid1, Omar A Sharaf Eldein5, Gehan Attia Alsawah6, Sohier Yahia7, Iman M Fawzy8. 1. a Pediatric Nephrology Unit, Mansoura University Children's Hospital , Mansoura , Egypt . 2. b Clinical Immunology Unit, Clinical Pathology Department & Mansoura Research Center for Cord Stem Cells (MARC_CSC), Faculty of Medicine, Mansoura University , Mansoura , Egypt . 3. c Rheumatology and Rehabilitation Department, Mansoura University Hospital , Mansoura , Egypt . 4. d Dermatology and Andrology Department, Mansoura Faculty of Medicine , Mansoura , Egypt . 5. e Clinical Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University , Mansoura , Egypt . 6. f Pediatric Cardiology Unit and. 7. g Genetics Unit, Mansoura University Children's Hospital , Mansoura , Egypt , and. 8. h Laboratory Medicine Department , Mansoura Fever Hospital, Ministry of Health , Mansoura , Egypt.
Abstract
UNLABELLED: There are no reports about the association of interleukin (IL)-17A and IL-17F gene polymorphism and susceptibility to pediatric systemic lupus erythematosus (pSLE). OBJECTIVE: To examine the possible role of IL-17A rs2275913, IL-17F rs763780 and rs2397084 polymorphisms as risk factors for pSLE in a cohort of Egyptian children and to investigate their association with the clinico-pathological features including lupus nephritis (LN). METHODS: Typing of IL-17A and IL-17F polymorphisms was done using restriction fragment length polymorphism for 115 children with SLE and 259 age- and sex-matched healthy controls. RESULTS: No significant differences were found between pSLE patients and healthy controls for the allele and genotype frequencies of IL-17A rs2275913, IL-17F rs763780 and rs2397084 (p > 0.05). However, the combined genotype GGAGAA and the haplotype GGA had significant association with pSLE (pc = 0.042 and <0.001, respectively). The AA genotype of IL-17F rs763780 is more frequent in female patients (p = 0.002) and the AA genotype of IL-17F rs2397084 is more associated with positivity of ds-DNA (p = 0.007). No more associations were found for the demographic and clinical data of pSLE patients including risk of LN development, risk of non-remission, overall survival, activity and chronicity indices. CONCLUSION: The GGAGAA combined genotype and the GGA haplotype of IL-17A rs2275913, IL-17F rs763780 and rs2397084 can be considered risk factors for the development of SLE in Egyptian children. IL-17A rs2275913, IL-17F rs763780 and rs2397084 are not related to the LN development, SLE disease activity or overall survival.
UNLABELLED: There are no reports about the association of interleukin (IL)-17A and IL-17F gene polymorphism and susceptibility to pediatric systemic lupus erythematosus (pSLE). OBJECTIVE: To examine the possible role of IL-17Ars2275913, IL-17Frs763780 and rs2397084 polymorphisms as risk factors for pSLE in a cohort of Egyptian children and to investigate their association with the clinico-pathological features including lupus nephritis (LN). METHODS: Typing of IL-17A and IL-17F polymorphisms was done using restriction fragment length polymorphism for 115 children with SLE and 259 age- and sex-matched healthy controls. RESULTS: No significant differences were found between pSLE patients and healthy controls for the allele and genotype frequencies of IL-17Ars2275913, IL-17Frs763780 and rs2397084 (p > 0.05). However, the combined genotype GGAGAA and the haplotype GGA had significant association with pSLE (pc = 0.042 and <0.001, respectively). The AA genotype of IL-17Frs763780 is more frequent in female patients (p = 0.002) and the AA genotype of IL-17Frs2397084 is more associated with positivity of ds-DNA (p = 0.007). No more associations were found for the demographic and clinical data of pSLE patients including risk of LN development, risk of non-remission, overall survival, activity and chronicity indices. CONCLUSION: The GGAGAA combined genotype and the GGA haplotype of IL-17Ars2275913, IL-17Frs763780 and rs2397084 can be considered risk factors for the development of SLE in Egyptian children. IL-17Ars2275913, IL-17Frs763780 and rs2397084 are not related to the LN development, SLE disease activity or overall survival.