PURPOSE: The aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF). METHODS: The control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry. RESULTS: We observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance. CONCLUSION: This analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.
PURPOSE: The aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF). METHODS: The control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry. RESULTS: We observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance. CONCLUSION: This analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.
Entities:
Keywords:
Endometrioma; Endometriosis; Follicular fluid; IVF; Mass spectrometry, lipids; Metabolomics
Authors: Naoko Sasamoto; Oana A Zeleznik; Allison F Vitonis; Stacey A Missmer; Marc R Laufer; Julian Avila-Pacheco; Clary B Clish; Kathryn L Terry Journal: Fertil Steril Date: 2022-03-30 Impact factor: 7.490
Authors: Vitaliy V Chagovets; Zhihao Wang; Alexey S Kononikhin; Natalia L Starodubtseva; Anna Borisova; Dinara Salimova; Igor A Popov; Andrey V Kozachenko; Konstantin Chingin; Huanwen Chen; Vladimir E Frankevich; Leila V Adamyan; Gennady T Sukhikh Journal: Sci Rep Date: 2017-05-31 Impact factor: 4.379
Authors: Pierre-Emmanuel Bouet; Juan-Manuel Chao de la Barca; Lisa Boucret; Philippe Descamps; Guillaume Legendre; Hady El Hachem; Simon Blanchard; Pascale Jeannin; Pascal Reynier; Pascale May-Panloup Journal: J Clin Med Date: 2020-05-01 Impact factor: 4.241