Literature DB >> 2649686

Crystallization and preliminary X-ray analysis of the human c-H-ras-oncogene product p21 complexed with GTP analogues.

A Scherer1, J John, R Linke, R S Goody, A Wittinghofer, E F Pai, K C Homes.   

Abstract

The catalytic domain (amino acid residues 1 to 166) of the human ras-oncogene product p21 complexed with the GTP analogues beta,gamma-imido-GTP (GMPPNP), beta,gamma-methylene-GTP (GMPPCP), and guanosine-5'-(gamma-thiotriphosphate) (GTP gamma S) have been been crystallized. Crystals of the GMPPNP and GMPPCP complexes are well suited for high resolution X-ray crystallography. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) with unit cell axes a=b=40.3 A and c = 162.2 A.

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Year:  1989        PMID： 2649686     DOI： 10.1016/0022-2836(89)90540-8

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  12 in total

1.  A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.

Authors:  Björn U Klink; Roger S Goody; Axel J Scheidig
Journal:  Biophys J       Date:  2006-05-12       Impact factor: 4.033

2.  Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal.

Authors:  M G Rudolph; A Wittinghofer; I R Vetter
Journal:  Protein Sci       Date:  1999-04       Impact factor: 6.725

3.  Guanosine triphosphatase stimulation of oncogenic Ras mutants.

Authors:  M R Ahmadian; T Zor; D Vogt; W Kabsch; Z Selinger; A Wittinghofer; K Scheffzek
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-08       Impact factor: 11.205

4.  The effect of 17O on the relaxation of an amide proton within a hydrogen bond.

Authors:  C J Halkides; A G Redfield
Journal:  J Biomol NMR       Date:  1995-06       Impact factor: 2.835

5.  Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching.

Authors:  Rane A Harrison; Jia Lu; Martin Carrasco; John Hunter; Anuj Manandhar; Sudershan Gondi; Kenneth D Westover; John R Engen
Journal:  J Mol Biol       Date:  2016-10-14       Impact factor: 5.469

6.  Biochemical and crystallographic characterization of a complex of c-Ha-ras p21 and caged GTP with flash photolysis.

Authors:  I Schlichting; G Rapp; J John; A Wittinghofer; E F Pai; R S Goody
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

7.  Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

Authors:  E F Pai; U Krengel; G A Petsko; R S Goody; W Kabsch; A Wittinghofer
Journal:  EMBO J       Date:  1990-08       Impact factor: 11.598

8.  Mutations of Ha-ras p21 that define important regions for the molecular mechanism of the SDC25 C-domain, a guanine nucleotide dissociation stimulator.

Authors:  M Y Mistou; E Jacquet; P Poullet; H Rensland; P Gideon; I Schlichting; A Wittinghofer; A Parmeggiani
Journal:  EMBO J       Date:  1992-07       Impact factor: 11.598

9.  K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions.

Authors:  Jonathan M Ostrem; Ulf Peters; Martin L Sos; James A Wells; Kevan M Shokat
Journal:  Nature       Date:  2013-11-20       Impact factor: 49.962

Review 10.  Three-dimensional structure of p21 in the active conformation and analysis of an oncogenic mutant.

Authors:  F Wittinghofer; U Krengel; J John; W Kabsch; E F Pai
Journal:  Environ Health Perspect       Date:  1991-06       Impact factor: 9.031
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