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Literature DB >> 2648397

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

Abstract

We prepared a set of temperature-sensitive mutants of the EcoRI endonuclease. Under semipermissive conditions, Escherichia coli strains bearing these alleles form poorly growing colonies in which intracellular substrates are cleaved at EcoRI sites and the SOS DNA repair response is induced. Strains defective in SOS induction (lexA3 mutant) or SOS induction and recombination (recA56 and recB21 mutants) are not more sensitive to this in vivo DNA scission, whereas strains deficient in DNA ligase (lig4 and lig ts7 mutants) are extremely sensitive. We conclude that although DNA scission induces the SOS response, neither this induction nor recombination are required for repair. DNA ligase is necessary and may be sufficient to repair EcoRI-mediated DNA breaks in the E. coli chromosome.

Entities: Species

Mesh：

Substances：
Deoxyribonuclease EcoRI

Year: 1989 PMID： 2648397 PMCID： PMC286896 DOI： 10.1073/pnas.86.7.2281

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase.

Authors: E B Konrad; P Modrich; I R Lehman
Journal: J Mol Biol Date: 1973-07-15 Impact factor: 5.469

2. Genetics and function of DNA ligase in Escherichia coli.

Authors: M M Gottesman; M L Hicks; M Gellert
Journal: J Mol Biol Date: 1973-07-15 Impact factor: 5.469

Review 3. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

Authors: G C Walker
Journal: Microbiol Rev Date: 1984-03

4. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.

Authors: S K Shapira; J Chou; F V Richaud; M J Casadaban
Journal: Gene Date: 1983-11 Impact factor: 3.688

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

1. Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase.

2. Genetics and function of DNA ligase in Escherichia coli.

Review 3. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

4. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.

5. Activation of Chi, a recombinator, by the action of an endonuclease at a distant site.

6. Positive-selection cloning vehicle useful for overproduction of hybrid proteins.

7. A genetic system for analysis of staphylococcal nuclease.

8. gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.

9. The filamentous phage (Ff) as vectors for recombinant DNA--a review.

10. Separate sites for binding and nicking of bacteriophage lambda DNA by terminase.

1. Cellular responses to postsegregational killing by restriction-modification genes.

2. Recognition of native DNA methylation by the PvuII restriction endonuclease.

3. Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease.

Review 4. Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution.

5. Cloning and characterization of genes for the PvuI restriction and modification system.

6. Topological domain structure of the Escherichia coli chromosome.

7. One recognition sequence, seven restriction enzymes, five reaction mechanisms.

8. Multiple components are involved in the efficient joining of double stranded DNA breaks in human cell extracts.

9. Promiscuous restriction is a cellular defense strategy that confers fitness advantage to bacteria.

10. The effect of recA mutation on the expression of EcoKI and EcoR124I hsd genes cloned in a multicopy plasmid.