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Literature DB >> 6302074

Positive-selection cloning vehicle useful for overproduction of hybrid proteins.

Abstract

Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.

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Year: 1983 PMID： 6302074 PMCID： PMC217560 DOI： 10.1128/jb.154.2.1005-1008.1983

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. EcoRI methylase. Physical and catalytic properties of the homogeneous enzyme.

Authors: R A Rubin; P Modrich
Journal: J Biol Chem Date: 1977-10-25 Impact factor: 5.157

4. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.

Authors: F W Studier
Journal: J Mol Biol Date: 1973-09-15 Impact factor: 5.469

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids.

Authors: D Dean
Journal: Gene Date: 1981-10 Impact factor: 3.688

7. Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

Authors: P J Greene; M Gupta; H W Boyer; W E Brown; J M Rosenberg
Journal: J Biol Chem Date: 1981-03-10 Impact factor: 5.157

8. DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

Authors: A K Newman; R A Rubin; S H Kim; P Modrich
Journal: J Biol Chem Date: 1981-03-10 Impact factor: 5.157

9. Translational control of bacteriophage f1 gene II and gene X proteins by gene V protein.

Authors: T S Yen; R E Webster
Journal: Cell Date: 1982-06 Impact factor: 41.582

10. Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd.

Authors: E Beck; B Zink
Journal: Gene Date: 1981-12 Impact factor: 3.688

12 in total

1. Cloning and characterization of genes for the PvuI restriction and modification system.

Authors: M D Smith; M Longo; G F Gerard; D K Chatterjee
Journal: Nucleic Acids Res Date: 1992-11-11 Impact factor: 16.971

2. Recombination and replication of plasmid-like derivatives of a short section of the mitochondrial chromosome of Neurospora crassa.

Authors: S R Gross; P H Levine; S Metzger; G Glaser
Journal: Genetics Date: 1989-04 Impact factor: 4.562

3. Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.

Authors: S S Su; P Modrich
Journal: Proc Natl Acad Sci U S A Date: 1986-07 Impact factor: 11.205

4. Molecular evolution of streptococcal M protein: cloning and nucleotide sequence of the type 24 M protein gene and relation to other genes of Streptococcus pyogenes.

Authors: A R Mouw; E H Beachey; V Burdett
Journal: J Bacteriol Date: 1988-02 Impact factor: 3.490