Literature DB >> 2648132

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

M Winey1, I Edelman, M R Culbertson.   

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

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Year:  1989        PMID： 2648132      PMCID： PMC362177          DOI： 10.1128/mcb.9.1.329-331.1989

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  13 in total

Review 1.  Splicing of messenger RNA precursors.

Authors:  R A Padgett; P J Grabowski; M M Konarska; S Seiler; P A Sharp
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

2.  Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.

Authors:  C L Peebles; P Gegenheimer; J Abelson
Journal:  Cell       Date:  1983-02       Impact factor: 41.582

3.  Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.

Authors:  H Swerdlow; C Guthrie
Journal:  J Biol Chem       Date:  1984-04-25       Impact factor: 5.157

4.  Mechanism of action of a yeast RNA ligase in tRNA splicing.

Authors:  C L Greer; C L Peebles; P Gegenheimer; J Abelson
Journal:  Cell       Date:  1983-02       Impact factor: 41.582

5.  A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.

Authors:  M D Rose; P Novick; J H Thomas; D Botstein; G R Fink
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Most of the yeast genomic sequences are not essential for cell growth and division.

Authors:  M G Goebl; T D Petes
Journal:  Cell       Date:  1986-09-26       Impact factor: 41.582

7.  Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.

Authors:  R C Ogden; M C Lee; G Knapp
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

10.  Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.

Authors:  M C Lee; G Knapp
Journal:  J Biol Chem       Date:  1985-03-10       Impact factor: 5.157
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  3 in total

1.  In vivo pre-tRNA processing in Saccharomyces cerevisiae.

Authors:  J P O'Connor; C L Peebles
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

2.  Mutations in the anticodon stem affect removal of introns from pre-tRNA in Saccharomyces cerevisiae.

Authors:  L Mathison; M Winey; C Soref; M R Culbertson; G Knapp
Journal:  Mol Cell Biol       Date:  1989-10       Impact factor: 4.272

3.  Exceptional codon recognition by the glutamine tRNAs in Saccharomyces cerevisiae.

Authors:  I Edelman; M R Culbertson
Journal:  EMBO J       Date:  1991-06       Impact factor: 11.598
  3 in total

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