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Literature DB >> 2646593

A catalytic 13-mer ribozyme.

Abstract

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.

Entities: Chemical Gene Species

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Year: 1989 PMID： 2646593 PMCID： PMC331809 DOI： 10.1093/nar/17.4.1371

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Self-cleaving transcripts of satellite DNA from the newt.

Authors: L M Epstein; J G Gall
Journal: Cell Date: 1987-02-13 Impact factor: 41.582

2. Structural and ionic requirements for self-cleavage of virusoid RNAs and trans self-cleavage of viroid RNA.

Authors: A C Forster; A C Jeffries; C C Sheldon; R H Symons
Journal: Cold Spring Harb Symp Quant Biol Date: 1987

3. Cleavage of specific sites of RNA by designed ribozymes.

Authors: M Koizumi; S Iwai; E Ohtsuka
Journal: FEBS Lett Date: 1988-11-07 Impact factor: 4.124

4. Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

Authors: M Koizumi; S Iwai; E Ohtsuka
Journal: FEBS Lett Date: 1988-02-15 Impact factor: 4.124

5. Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site.

Authors: A C Forster; R H Symons
Journal: Cell Date: 1987-07-03 Impact factor: 41.582

Review 6. A replication cycle for viroids and other small infectious RNA's.

Authors: A D Branch; H D Robertson
Journal: Science Date: 1984-02-03 Impact factor: 47.728

7. A nonlinear regression program for small computers.

Authors: R G Duggleby
Journal: Anal Biochem Date: 1981-01-01 Impact factor: 3.365

8. Chrysanthemum stunt viroid: primary sequence and secondary structure.

Authors: J Haseloff; R H Symons
Journal: Nucleic Acids Res Date: 1981-06-25 Impact factor: 16.971

9. Self-cleaving viroid and newt RNAs may only be active as dimers.

Authors: A C Forster; C Davies; C C Sheldon; A C Jeffries; R H Symons
Journal: Nature Date: 1988-07-21 Impact factor: 49.962

10. Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.

Authors: C J Hutchins; P D Rathjen; A C Forster; R H Symons
Journal: Nucleic Acids Res Date: 1986-05-12 Impact factor: 16.971

29 in total

1. Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET).

Authors: K K Singh; R Parwaresch; G Krupp
Journal: RNA Date: 1999-10 Impact factor: 4.942

9. Use of the 1-(2-fluorophenyl)-4-methoxypiperidin-4-yl (Fpmp) and related protecting groups in oligoribonucleotide synthesis: stability of internucleotide linkages to aqueous acid.

Authors: D C Capaldi; C B Reese
Journal: Nucleic Acids Res Date: 1994-06-25 Impact factor: 16.971

10. Target sequence-specific inhibition of HIV-1 replication by ribozymes directed to tat RNA.

Authors: L Q Sun; L Wang; W L Gerlach; G Symonds
Journal: Nucleic Acids Res Date: 1995-08-11 Impact factor: 16.971

A catalytic 13-mer ribozyme.

1. Self-cleaving transcripts of satellite DNA from the newt.

2. Structural and ionic requirements for self-cleavage of virusoid RNAs and trans self-cleavage of viroid RNA.

3. Cleavage of specific sites of RNA by designed ribozymes.

4. Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.

5. Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site.

Review 6. A replication cycle for viroids and other small infectious RNA's.

7. A nonlinear regression program for small computers.

8. Chrysanthemum stunt viroid: primary sequence and secondary structure.

9. Self-cleaving viroid and newt RNAs may only be active as dimers.

10. Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.

1. Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET).

Review 2. Recent advances in the elucidation of the mechanisms of action of ribozymes.

3. A ribozyme with DNA in the hybridising arms displays enhanced cleavage ability.

4. Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity.

5. Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.

6. Alternative modes of self-cleavage by newt satellite 2 transcripts.

7. Alternative hammerhead structures in the self-cleavage of avocado sunblotch viroid RNAs.

8. An oligodeoxyribonucleotide that supports catalytic activity in the hammerhead ribozyme domain.

9. Use of the 1-(2-fluorophenyl)-4-methoxypiperidin-4-yl (Fpmp) and related protecting groups in oligoribonucleotide synthesis: stability of internucleotide linkages to aqueous acid.

10. Target sequence-specific inhibition of HIV-1 replication by ribozymes directed to tat RNA.