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Literature DB >> 2460373

Cleavage of specific sites of RNA by designed ribozymes.

Abstract

Two ribozymes were designed for site-specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19-mer and a 13-mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.

Entities: Chemical

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Year: 1988 PMID： 2460373 DOI： 10.1016/0014-5793(88)80935-9

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

32 in total

1. Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET).

Authors: K K Singh; R Parwaresch; G Krupp
Journal: RNA Date: 1999-10 Impact factor: 4.942

2. Small, efficient hammerhead ribozymes.

Authors: M J McCall; P Hendry; A A Mir; J Conaty; G Brown; T J Lockett
Journal: Mol Biotechnol Date: 2000-01 Impact factor: 2.695

3. Comparison of the specificities and catalytic activities of hammerhead ribozymes and DNA enzymes with respect to the cleavage of BCR-ABL chimeric L6 (b2a2) mRNA.

Authors: T Kuwabara; M Warashina; T Tanabe; K Tani; S Asano; K Taira
Journal: Nucleic Acids Res Date: 1997-08-01 Impact factor: 16.971

4. A ribozyme with DNA in the hybridising arms displays enhanced cleavage ability.

Authors: P Hendry; M J McCall; F S Santiago; P A Jennings
Journal: Nucleic Acids Res Date: 1992-11-11 Impact factor: 16.971

5. Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

Authors: L Mazzolini; M Axelos; N Lescure; P Yot
Journal: Plant Mol Biol Date: 1992-11 Impact factor: 4.076

Cleavage of specific sites of RNA by designed ribozymes.

1. Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET).

2. Small, efficient hammerhead ribozymes.

3. Comparison of the specificities and catalytic activities of hammerhead ribozymes and DNA enzymes with respect to the cleavage of BCR-ABL chimeric L6 (b2a2) mRNA.

4. A ribozyme with DNA in the hybridising arms displays enhanced cleavage ability.

5. Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

6. Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity.

7. Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.

8. A catalytic 13-mer ribozyme.

9. Alternative hammerhead structures in the self-cleavage of avocado sunblotch viroid RNAs.

10. Minimal sequence requirements for ribozyme activity.