| Literature DB >> 26463383 |
Charles Danan1, Sudhir Manickavel1, Markus Hafner2.
Abstract
During post-transcriptional gene regulation (PTGR), RNA binding proteins (RBPs) interact with all classes of RNA to control RNA maturation, stability, transport, and translation. Here, we describe Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a transcriptome-scale method for identifying RBP binding sites on target RNAs with nucleotide-level resolution. This method is readily applicable to any protein directly contacting RNA, including RBPs that are predicted to bind in a sequence- or structure-dependent manner at discrete RNA recognition elements (RREs), and those that are thought to bind transiently, such as RNA polymerases or helicases.Entities:
Keywords: Binding site; Cross-linking and immunoprecipitation (CLIP); Noncoding RNA; Photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation (PAR-CLIP); Posttranscriptional gene regulation (PTGR); RNA; RNA recognition element (RRE); RNA-binding protein (RBP); mRNA
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Year: 2016 PMID： 26463383 PMCID： PMC5142217 DOI： 10.1007/978-1-4939-3067-8_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745