Literature DB >> 26436126

Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam.

Mousumi Bora1, Durlav Prasad Bora1, Nagendra Nath Barman1, Biswajyoti Borah1, Padma Lochan Bora1, Archana Talukdar2, Shantanu Tamuly3.   

Abstract

Outbreaks of contagious ecthyma (caused by a Parapox virus) in goats were investigated in 6 districts of Assam, a north eastern state of India. Diagnosis of the disease was carried out employing both standard virological as well as molecular methods. Four representative isolates from different places were selected for phylogenetic analysis. The major envelop protein (B2L) of Orf virus was targeted for molecular analysis. The sequencing and phylogenetic analysis of the selected sequences at nucleotide level revealed that the Orf virus isolates were closely related to each other (97.6-100 %) and showed highest similarity to the Orf virus isolate 82/04 (98.4 %), reported from Shahjahanpur, India. The data will provide an insight in transmission of the virus from northern to North eastern part of the country.

Entities:  

Keywords:  Assam; B2L gene; Contagious ecthyma; Goat; Orf virus; Phylogenetic analysis

Year:  2015        PMID： 26436126      PMCID： PMC4585050          DOI： 10.1007/s13337-015-0255-y

Source DB:  PubMed          Journal:  Virusdisease        ISSN： 2347-3584


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