| Literature DB >> 26645035 |
Ashwaq Ahmed Abdullah1, Muhammad Farid Bin Ismail2, Krishnan Nair Balakrishnan2, Jamilu Abubakar Bala2, Homayoun Hani2, Yusuf Abba2, Mohd Kamaruddin Awang Isa2, Faez Firdaus Jesse Abdullah3, Siti Suri Arshad2, Zeenatul Allaudin Nazariah4, Rasedee Abdullah4, Noordin Mohamed Mustapha2, Mohd-Azmi Mohd-Lila1.
Abstract
Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally.Entities:
Keywords: Contagious ecthyma; Goat; Orf virus; Parapoxvirus; Sore-mouth infection
Year: 2015 PMID： 26645035 PMCID： PMC4663714 DOI： 10.1007/s13337-015-0278-4
Source DB: PubMed Journal: Virusdisease ISSN： 2347-3584