Aleksandar Jakovljevic1, Miroslav Andric2, Aleksandra Knezevic3, Ivan Soldatovic4, Nadja Nikolic5, Danijela Karalic3, Jelena Milasin5. 1. School of Dental Medicine, Clinic for Oral Surgery and Implantology, University of Belgrade, Belgrade, Serbia. Electronic address: dr.sasuli@hotmail.com. 2. School of Dental Medicine, Clinic for Oral Surgery and Implantology, University of Belgrade, Belgrade, Serbia. 3. Faculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade, Belgrade, Serbia. 4. Faculty of Medicine, University of Belgrade, Belgrade, Serbia. 5. School of Dental Medicine, Institute of Biology and Human Genetics, University of Belgrade, Belgrade, Serbia.
Abstract
INTRODUCTION: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. METHODS: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. RESULTS: EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). CONCLUSIONS: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.
INTRODUCTION: Different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) possess specific pathogenic abilities because of various interactions with the host's immune system and differences in cell tropism. The aim of this study was to determine the distribution of HCMV and EBV genotypes in apical periodontitis lesions in relation to their clinical and histopathologic features. METHODS: One hundred samples of apical periodontitis lesions and 25 control samples (healthy pulp tissue) were collected. The presence of HCMV glycoprotein B (gB) and EBV nuclear antigen-2 genotypes was analyzed by nested polymerase chain reaction and restriction fragment length polymorphisms analysis. RESULTS:EBV and HCMV were detected in apical periodontitis lesions at significantly higher frequencies than in healthy pulp controls (P = .020 and P = .020, respectively). HCMV gB type II was significantly more frequent compared with gB type I in the examined groups (P = .036). No HCMV gB type III or IV products were found. In both periapical lesions and controls, EBV-1 occurred more often compared with EBV-2 (P = .001). Dual EBV and HCMV coinfection was more frequently detected in large-size periapical lesions (P = .038). CONCLUSIONS: Both HCMV and EBV are associated with inflammatory processes of periapical bone destruction. HCMV gB type II and EBV-1 are the most prevalent genotypes in apical periodontitis lesions.
Authors: Camila Freze Baez; Flavia Savassi-Ribas; Wilker Menezes da Rocha; Stéphanie G S Almeida; Marianna T V Gonçalves; Maria Angelica A M Guimarães; Silvia Maria B Cavalcanti; Rafael B Varella Journal: Rev Inst Med Trop Sao Paulo Date: 2016-07-11 Impact factor: 1.846
Authors: Hsuan-Yuan Wang; Sarah M Valencia; Susanne P Pfeifer; Jeffrey D Jensen; Timothy F Kowalik; Sallie R Permar Journal: Viruses Date: 2021-06-09 Impact factor: 5.818