Aleksandar Jakovljevic1, Miroslav Andric2, Aleksandra Knezevic3, Biljana Milicic4, Katarina Beljic-Ivanovic5, Neda Perunovic6, Nadja Nikolic7, Jelena Milasin7. 1. Clinic of Oral Surgery and Implantology, School of Dental Medicine, University of Belgrade, dr. Subotica 4, Belgrade, 11 000, Serbia. dr.sasuli@hotmail.com. 2. Clinic of Oral Surgery and Implantology, School of Dental Medicine, University of Belgrade, dr. Subotica 4, Belgrade, 11 000, Serbia. 3. Department of Virology, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, dr. Subotica 1, Belgrade, 11 000, Serbia. 4. Department of Medical Statistics and Informatics, School of Dental Medicine, University of Belgrade, Dr. Subotica 1, Belgrade, 11 000, Serbia. 5. Clinic of Restorative Dentistry and Endodontics, School of Dental Medicine, University of Belgrade, Rankeova 4, Belgrade, 11 000, Serbia. 6. Clinic of Periodontology and Oral Medicine, School of Dental Medicine, University of Belgrade, Dr. Subotica 4, Belgrade, 11 000, Serbia. 7. Department of Biology and Human Genetics, School of Dental Medicine, University of Belgrade, Dr. Subotica 1, Belgrade, 11 000, Serbia.
Abstract
OBJECTIVE: The aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. MATERIALS AND METHODS: Fifty samples obtained with paper points (30 from pericoronitis and 20 controls) were subjected to polymerase chain reaction (PCR) analysis. A single-stage and nested PCR assays were used to detect herpesviruses: human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) and six periodontopathic anaerobic bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, Treponema denticola, and Tannarella forsythia. RESULTS: Pericoronitis samples harbored HCMV and EBV at significantly higher rates than the control group (70 vs. 40 % and 46.7 vs. 15 %, P = 0.035, P = 0.021, respectively). P. micra and T. forsythia (66.7 vs. 0 %, and 40 vs. 10 %, P = 0.001, P = 0.021, respectively) were significantly more common in pericoronitis compared to the control group. Multivariate logistic regression analysis showed that the presence of T. forsythia was associated with pericoronitis development (OR 7.3, 95 % CI, 1.2-43.2, P = 0.028). CONCLUSION: The occurrence of HCVM and EBV extends our previous knowledge on microbiota in pericoronitis. These PCR-based findings demonstrated that bacterial and viral DNA occurred concomitantly in pericoronitis samples. T. forsythia appeared to be significantly associated with pericoronitis development in the examined sample. CLINICAL RELEVANCE: Herpesviral-bacterial co-infections might exacerbate the progression of pericoronitis.
OBJECTIVE: The aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. MATERIALS AND METHODS: Fifty samples obtained with paper points (30 from pericoronitis and 20 controls) were subjected to polymerase chain reaction (PCR) analysis. A single-stage and nested PCR assays were used to detect herpesviruses: human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) and six periodontopathic anaerobic bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, Treponema denticola, and Tannarella forsythia. RESULTS: Pericoronitis samples harbored HCMV and EBV at significantly higher rates than the control group (70 vs. 40 % and 46.7 vs. 15 %, P = 0.035, P = 0.021, respectively). P. micra and T. forsythia (66.7 vs. 0 %, and 40 vs. 10 %, P = 0.001, P = 0.021, respectively) were significantly more common in pericoronitis compared to the control group. Multivariate logistic regression analysis showed that the presence of T. forsythia was associated with pericoronitis development (OR 7.3, 95 % CI, 1.2-43.2, P = 0.028). CONCLUSION: The occurrence of HCVM and EBV extends our previous knowledge on microbiota in pericoronitis. These PCR-based findings demonstrated that bacterial and viral DNA occurred concomitantly in pericoronitis samples. T. forsythia appeared to be significantly associated with pericoronitis development in the examined sample. CLINICAL RELEVANCE: Herpesviral-bacterial co-infections might exacerbate the progression of pericoronitis.
Authors: Aleksandar Jakovljevic; Miroslav Andric; Aleksandra Knezevic; Ivan Soldatovic; Nadja Nikolic; Danijela Karalic; Jelena Milasin Journal: J Endod Date: 2015-10-02 Impact factor: 4.171