| Literature DB >> 2643102 |
R G Goodwin1, S Lupton, A Schmierer, K J Hjerrild, R Jerzy, W Clevenger, S Gillis, D Cosman, A E Namen.
Abstract
A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.Entities:
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Year: 1989 PMID： 2643102 PMCID： PMC286452 DOI： 10.1073/pnas.86.1.302
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205