| Literature DB >> 26428033 |
Tomaž Einfalt1, Roland Goers1,2, Ionel Adrian Dinu1, Adrian Najer1, Mariana Spulber1, Ozana Onaca-Fischer1, Cornelia G Palivan1.
Abstract
The development of advanced stimuli-responsive systems for medicine, catalysis, or technology requires compartmentalized reaction spaces with triggered activity. Only very few stimuli-responsive systems preserve the compartment architecture, and none allows a triggered activity in situ. We present here a biomimetic strategy to molecular transmembrane transport by engineering synthetic membranes equipped with channel proteins so that they are stimuli-responsive. Nanoreactors with triggered activity were designed by simultaneously encapsulating an enzyme inside polymer compartments, and inserting protein "gates" in the membrane. The outer membrane protein F (OmpF) porin was chemically modified with a pH-responsive molecular cap to serve as "gate" producing pH-driven molecular flow through the membrane and control the in situ enzymatic activity. This strategy provides complex reaction spaces necessary in "smart" medicine and for biomimetic engineering of artificial cells.Entities:
Keywords: Nanotechnology; confinement; membrane permeability; membrane protein; polymer chemistry; polymersomes
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Year: 2015 PMID： 26428033 DOI： 10.1021/acs.nanolett.5b03386
Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189