Literature DB >> 26373293

Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system.

Weiguang Shan1, Jiaping Li1, Ying Fang2, Xuan Wang2, Danxia Gu2, Rong Zhang3.   

Abstract

A rapid, sensitive, and accurate Vitek MS assay was developed to distinguish clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from clinical isolates of methicillin-sensitive Staphylococcus aureus (MSSA) by developing an in-house knowledgebase of SuperSpectra. Three unique peaks, including peaks at 2305.6 and 3007.3 Da specific to MRSA, and 6816.7 Da specific to MSSA, were selected for differentiating MRSA and MSSA. This assay accurately identified 84 and 91% of clinical MRSA and MSSA strains out of the total 142 clinically acquired S. aureus strains that were tested. This method will greatly improve the efficiency of single clinical sample identification of MRSA, thereby facilitating a reduction in the transmission of MRSA in clinical settings.

Entities:  

Mesh：

Year:  2015        PMID： 26373293     DOI： 10.1007/s00284-015-0913-8

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  12 in total

1.  Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Authors:  Delphine Martiny; Laurent Busson; Ingrid Wybo; Rachid Ait El Haj; Anne Dediste; Olivier Vandenberg
Journal:  J Clin Microbiol       Date:  2012-02-08       Impact factor: 5.948

2.  Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak.

Authors:  Paul M Griffin; Gareth R Price; Jacqueline M Schooneveldt; Sanmarié Schlebusch; Martyn H Tilse; Tess Urbanski; Brett Hamilton; Deon Venter
Journal:  J Clin Microbiol       Date:  2012-06-27       Impact factor: 5.948

3.  A rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for single-plasmid tracking in an outbreak of carbapenem-resistant Enterobacteriaceae.

Authors:  Anna F Lau; Honghui Wang; Rebecca A Weingarten; Steven K Drake; Anthony F Suffredini; Mark K Garfield; Yong Chen; Marjan Gucek; Jung-Ho Youn; Frida Stock; Hanna Tso; Jim DeLeo; James J Cimino; Karen M Frank; John P Dekker
Journal:  J Clin Microbiol       Date:  2014-05-21       Impact factor: 5.948

4.  Enzyme activated photodynamic therapy for methicillin-resistant Staphylococcus aureus infection both inv itro and in vivo.

Authors:  Xiu-Jun Fu; Yun-Qing Zhu; Yin-Bo Peng; You-Shuang Chen; Yi-Ping Hu; Hua-Xiang Lu; Wei-Rong Yu; Yong Fang; Jian-Zhong Du; Min Yao
Journal:  J Photochem Photobiol B       Date:  2014-05-02       Impact factor: 6.252

5.  Insufficient discriminatory power of MALDI-TOF mass spectrometry for typing of Enterococcus faecium and Staphylococcus aureus isolates.

Authors:  Peter Lasch; Carola Fleige; Maren Stämmler; Franziska Layer; Ulrich Nübel; Wolfgang Witte; Guido Werner
Journal:  J Microbiol Methods       Date:  2014-03-12       Impact factor: 2.363

6.  Culture conditions and sample preparation methods affect spectrum quality and reproducibility during profiling of Staphylococcus aureus with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors:  J E Goldstein; L Zhang; C M Borror; J V Rago; T R Sandrin
Journal:  Lett Appl Microbiol       Date:  2013-05-20       Impact factor: 2.858

7.  Characterization of Staphylococcus aureus isolated from clinical specimens by matrix assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors:  Ye Ru Wang; Qian Chen; Sheng Hui Cui; Feng Qin Li
Journal:  Biomed Environ Sci       Date:  2013-06       Impact factor: 3.118

8.  Pathogen characteristics reveal novel antibacterial approaches for interstitial lung disease.

Authors:  Hai-Wen Lu; Xiao-Bin Ji; Shuo Liang; Li-Chao Fan; Jiu-Wu Bai; Ke-Bing Chen; Yin Zhou; Hui-Ping Li; Jin-Fu Xu
Journal:  Pulm Pharmacol Ther       Date:  2014-04-01       Impact factor: 3.410

9.  Mass-spectrometry based minisequencing method for the rapid detection of drug resistance in Mycobacterium tuberculosis.

Authors:  Larisa N Ikryannikova; Maxim V Afanas'ev; Tat'yana A Akopian; Elena N Il'ina; Aleksey V Kuz'min; Elena E Larionova; Tat'yana G Smirnova; Larisa N Chernousova; Vadim M Govorun
Journal:  J Microbiol Methods       Date:  2007-05-31       Impact factor: 2.363

10.  The anti-repressor MecR2 promotes the proteolysis of the mecA repressor and enables optimal expression of β-lactam resistance in MRSA.

Authors:  Pedro Arêde; Catarina Milheiriço; Hermínia de Lencastre; Duarte C Oliveira
Journal:  PLoS Pathog       Date:  2012-07-26       Impact factor: 6.823
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.