| Literature DB >> 17602768 |
Larisa N Ikryannikova1, Maxim V Afanas'ev, Tat'yana A Akopian, Elena N Il'ina, Aleksey V Kuz'min, Elena E Larionova, Tat'yana G Smirnova, Larisa N Chernousova, Vadim M Govorun.
Abstract
A MALDI TOF MS based minisequencing method has been developed and applied for the analysis of rifampin (RIF)- and isoniazid (INH)-resistant M. tuberculosis strains. Eight genetic markers of RIF resistance-nucleotide polymorphisms located in RRDR of rpoB gene, and three of INH resistance including codon 315 of katG gene and -8 and -15 positions of the promoter region of fabG1-inhA operon were worked out. Based on the analysis of 100 M. tuberculosis strains collected from the Moscow region in 1997-2005 we deduced that 91% of RIF-resistant and 94% of INH-resistant strains can be identified using the technique suggested. The approach is rapid, reliable and allows to reveal the drug resistance of M. tuberculosis strains within 12 h after sample isolation.Entities:
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Year: 2007 PMID： 17602768 DOI： 10.1016/j.mimet.2007.05.015
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363