Heather Burks1, Jennifer Buckbinder2, Mary Francis-Hernandez3, Karine Chung4, Sami Jabara5, Kristin Bendikson6, Richard Paulson7. 1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. hburks@med.usc.edu. 2. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. Jennifer.Buckbinder@med.usc.edu. 3. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. Mary.Francis-Hernandez@med.usc.edu. 4. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. Karine.Chung@med.usc.edu. 5. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Kaiser Permanente, 4900 W. Sunset Boulevard, Los Angeles, CA, 90027, USA. Sami.Jabara@med.usc.edu. 6. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. Kristin.Bendikson@med.usc.edu. 7. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, Room 534, Los Angeles, CA, 90033, USA. rpaulson@med.usc.edu.
Abstract
PURPOSE: In fresh IVF cycles, embryos reaching the eight-cell stage on day 3 of development are thought to have a higher chance of implantation than those reaching this stage on day 4. To determine whether this difference persists after cryopreservation, we compared pregnancy and implantation rates between frozen embryo transfer (FET) cycles using delayed cleavage-stage embryos (cryopreserved day 4) and normal cleavage-stage embryos (cryopreserved day 3). METHODS: Participants underwent FET between 2008 and 2012 using embryos cryopreserved on either day 3 (n = 76) or day 4 (n = 48), depending on the length of time needed to achieve the eight-cell stage. All embryos, regardless of day of cryopreservation, were thawed and transferred on the 4th day of vaginal progesterone following endometrial preparation with oral estradiol. Chi-square and Mann-Whitney U tests were used to compare patient demographics and cycle outcomes. RESULTS: More women in the day 4 group had diminished ovarian reserve (44 vs 16 %, p = 0.003). Pregnancy outcomes in preceding fresh cycles were not different between the two groups. Pregnancy, implantation, and live birth rates following FET did not differ between the day 3 and day 4 groups. CONCLUSIONS: This is the first study to address outcomes using day 3 versus day 4 cryopreserved embryos. Despite a higher prevalence of diminished ovarian reserve (DOR) in the day 4 group, delayed cleavage-stage embryos utilized in FET cycles performed as well as embryos growing at the normal rate, suggesting delayed embryo development does not affect embryo implantation as long as endometrial synchrony is maintained.
PURPOSE: In fresh IVF cycles, embryos reaching the eight-cell stage on day 3 of development are thought to have a higher chance of implantation than those reaching this stage on day 4. To determine whether this difference persists after cryopreservation, we compared pregnancy and implantation rates between frozen embryo transfer (FET) cycles using delayed cleavage-stage embryos (cryopreserved day 4) and normal cleavage-stage embryos (cryopreserved day 3). METHODS:Participants underwent FET between 2008 and 2012 using embryos cryopreserved on either day 3 (n = 76) or day 4 (n = 48), depending on the length of time needed to achieve the eight-cell stage. All embryos, regardless of day of cryopreservation, were thawed and transferred on the 4th day of vaginal progesterone following endometrial preparation with oral estradiol. Chi-square and Mann-Whitney U tests were used to compare patient demographics and cycle outcomes. RESULTS: More women in the day 4 group had diminished ovarian reserve (44 vs 16 %, p = 0.003). Pregnancy outcomes in preceding fresh cycles were not different between the two groups. Pregnancy, implantation, and live birth rates following FET did not differ between the day 3 and day 4 groups. CONCLUSIONS: This is the first study to address outcomes using day 3 versus day 4 cryopreserved embryos. Despite a higher prevalence of diminished ovarian reserve (DOR) in the day 4 group, delayed cleavage-stage embryos utilized in FET cycles performed as well as embryos growing at the normal rate, suggesting delayed embryo development does not affect embryo implantation as long as endometrial synchrony is maintained.
Authors: Paul A Harris; Robert Taylor; Robert Thielke; Jonathon Payne; Nathaniel Gonzalez; Jose G Conde Journal: J Biomed Inform Date: 2008-09-30 Impact factor: 6.317
Authors: Laura van Loendersloot; Madelon van Wely; Fulco van der Veen; Patrick Bossuyt; Sjoerd Repping Journal: Reprod Biomed Online Date: 2014-05-15 Impact factor: 3.828
Authors: Peyman Saadat; Robert Boostanfar; Cristin C Slater; David E Tourgeman; Frank Z Stanczyk; Richard J Paulson Journal: Fertil Steril Date: 2004-07 Impact factor: 7.329
Authors: Eric D Levens; Brian W Whitcomb; Sasha Hennessy; Aidita N James; Belinda J Yauger; Frederick W Larsen Journal: Fertil Steril Date: 2008-01-07 Impact factor: 7.329
Authors: M Simopoulou; K Sfakianoudis; P Tsioulou; A Rapani; E Maziotis; P Giannelou; S Grigoriadis; A Pantou; K Nikolettos; N Vlahos; K Pantos; M Koutsilieris Journal: J Assist Reprod Genet Date: 2019-05-20 Impact factor: 3.412
Authors: Nayla J Bushaqer; Noor N Alkhudhairy; Ziyad M Alturaigi; Rowaida M Alhamad; Wadha A Mohawesh; Fatema E Alraka; Hisham A Ayyoub; M Dayou Nawal Journal: JBRA Assist Reprod Date: 2020-05-01