Zafer Orkun Toktaş1, Murat Şakir Ekşi2, Baran Yılmaz3, Mustafa Kemal Demir4, Serdar Özgen5, Türker Kılıç3, Deniz Konya3. 1. Department of Neurosurgery, Bahçeşehir University Medical Faculty, Çırağan Caddesi Osmanpaşa Mektebi Sokak No: 4-6, 34353, Beşiktaş, Istanbul, Turkey. drzafertoktas@gmail.com. 2. Department of Orthopedic Surgery, University of California at San Francisco, San Francisco, CA, USA. 3. Department of Neurosurgery, Bahçeşehir University Medical Faculty, Çırağan Caddesi Osmanpaşa Mektebi Sokak No: 4-6, 34353, Beşiktaş, Istanbul, Turkey. 4. Department of Radiology, Bahçeşehir University Medical Faculty, Istanbul, Turkey. 5. Department of Neurosurgery, Acıbadem University Medical Faculty, Istanbul, Turkey.
Abstract
PURPOSE: Several genomic loci have been previously found to be associated with intervertebral disc degeneration, so far. Data are mostly derived from northern European countries whereas data derived from Southern European Ancestor are limited. This study aimed to evaluate the association between radiological disease severity of lumbar disc degeneration and certain genetic loci in a sample of participants from Southern Europe. METHODS: Seventy-five patients with mild to severe lumbar disc degeneration and 25 healthy controls were enrolled into the study. In each subject, each lumbar intervertebral disc was separately examined to obtain a total radiological score for disease severity. In addition, single-nucleotide polymorphisms of predefined genetic samples were analyzed in all participants: COL1A1 Sp1, COL9a2 Trp2, COL9a3 Trp3, and VDR TaqI. RESULTS: Degeneration scores were significantly worse in cases with COL1A1 Sp1, COL9a3 Trp3, and VDR TaqI mutations; however, COL9a2 Trp2 mutation was not associated with a difference in the severity of disc degeneration. In addition, subjects with mutation in more than one gene sample (n = 20) had significantly worse degeneration scores than the remaining study participants (n = 80) (17.70 ± 2.72 vs. 21.81 ± 1.81, p < 0.001). CONCLUSION: Single-nucleotide polymorphisms occurring in COL1A1, COL9a3 and VDR genes seem to be associated with the development of lumbar disc degeneration in this cohort, possibly with even more pronounced association when multiple mutations are present in the same individual. By further prospective twin studies in associated genes and analyses of their relationship with environmental factors in an internationally sampled large cohort will make a more clear-minded conclusion about their association with disc degeneration, which would yield better appreciation and clinical planning of some predisposed people for these pathologies.
PURPOSE: Several genomic loci have been previously found to be associated with intervertebral disc degeneration, so far. Data are mostly derived from northern European countries whereas data derived from Southern European Ancestor are limited. This study aimed to evaluate the association between radiological disease severity of lumbar disc degeneration and certain genetic loci in a sample of participants from Southern Europe. METHODS: Seventy-five patients with mild to severe lumbar disc degeneration and 25 healthy controls were enrolled into the study. In each subject, each lumbar intervertebral disc was separately examined to obtain a total radiological score for disease severity. In addition, single-nucleotide polymorphisms of predefined genetic samples were analyzed in all participants: COL1A1 Sp1, COL9a2Trp2, COL9a3Trp3, and VDR TaqI. RESULTS: Degeneration scores were significantly worse in cases with COL1A1 Sp1, COL9a3Trp3, and VDR TaqI mutations; however, COL9a2Trp2 mutation was not associated with a difference in the severity of disc degeneration. In addition, subjects with mutation in more than one gene sample (n = 20) had significantly worse degeneration scores than the remaining study participants (n = 80) (17.70 ± 2.72 vs. 21.81 ± 1.81, p < 0.001). CONCLUSION: Single-nucleotide polymorphisms occurring in COL1A1, COL9a3 and VDR genes seem to be associated with the development of lumbar disc degeneration in this cohort, possibly with even more pronounced association when multiple mutations are present in the same individual. By further prospective twin studies in associated genes and analyses of their relationship with environmental factors in an internationally sampled large cohort will make a more clear-minded conclusion about their association with disc degeneration, which would yield better appreciation and clinical planning of some predisposed people for these pathologies.
Authors: P Paassilta; T Pihlajamaa; S Annunen; R G Brewton; B M Wood; C C Johnson; J Liu; Y Gong; M L Warman; D J Prockop; R Mayne; L Ala-Kokko Journal: J Biol Chem Date: 1999-08-06 Impact factor: 5.157
Authors: Jeffrey J T Jim; Noora Noponen-Hietala; Kenneth M C Cheung; Jürg Ott; Jaro Karppinen; Ahmad Sahraravand; Keith D K Luk; Shea-Ping Yip; Pak C Sham; You-Qiang Song; John C Y Leong; Kathryn S E Cheah; Leena Ala-Kokko; Danny Chan Journal: Spine (Phila Pa 1976) Date: 2005-12-15 Impact factor: 3.468
Authors: Tapio Videman; Janna Saarela; Jaakko Kaprio; Annu Näkki; Esko Levälahti; Kevin Gill; Leena Peltonen; Michele C Battié Journal: Arthritis Rheum Date: 2009-02
Authors: Pasi J Eskola; Per Kjaer; Iita M Daavittila; Svetlana Solovieva; Annaleena Okuloff; Joan S Sorensen; Niels Wedderkopp; Leena Ala-Kokko; Minna Männikkö; Jaro I Karppinen Journal: Int J Mol Epidemiol Genet Date: 2010-03-29
Authors: Jaro Karppinen; Eija Pääkkö; Petteri Paassilta; Jaana Lohiniva; Mauno Kurunlahti; Osmo Tervonen; Pentti Nieminen; Harald H H Göring; Antti Malmivaara; Heikki Vanharanta; Leena Ala-Kokko Journal: Radiology Date: 2003-02-19 Impact factor: 11.105
Authors: N A Morrison; J C Qi; A Tokita; P J Kelly; L Crofts; T V Nguyen; P N Sambrook; J A Eisman Journal: Nature Date: 1994-01-20 Impact factor: 49.962
Authors: Nikolay L Martirosyan; Arpan A Patel; Alessandro Carotenuto; M Yashar S Kalani; Evgenii Belykh; Corey T Walker; Mark C Preul; Nicholas Theodore Journal: Front Surg Date: 2016-11-21