Kayla N Ellefsen1,2, Jose Luiz da Costa1,3, Marta Concheiro4, Sebastien Anizan1, Allan J Barnes1, Sandrine Pirard1, David A Gorelick5, Marilyn A Huestis1. 1. Chemistry & Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. 2. Program in Toxicology, University of Maryland Baltimore, Baltimore, MD 21201, USA. 3. Forensic Toxicology & Chemistry Laboratory, Criminalistics Institute of Sao Paulo, Sao Paulo, SP, Brazil. 4. Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York, NY 10019, USA. 5. Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Abstract
BACKGROUND: DBS are an increasingly common clinical matrix. METHODS & RESULTS: Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood. CONCLUSION: DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.
BACKGROUND:DBS are an increasingly common clinical matrix. METHODS & RESULTS: Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood. CONCLUSION:DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.
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