BACKGROUND: At the start of their work, the European Bioanalysis Forum dried blood spots microsampling consortium did not form a dedicated team to investigate the spot homogeneity. However, two teams performed experiments that produced results relating to sample homogeneity. RESULTS: The data, which were produced via two different approaches (a radiolabeled and a nonradiolabeled approach), are highly complementary and demonstrate clear effects on sample inhomogeneity due to the substrate type, compound and hematocrit levels. CONCLUSION: The results demonstrate that sample inhomogeneity is a significant hurdle to the use of dried blood spots for regulated bioanalysis that should be investigated further in the method establishment phase if the whole spot is not sampled.
BACKGROUND: At the start of their work, the European Bioanalysis Forum dried blood spots microsampling consortium did not form a dedicated team to investigate the spot homogeneity. However, two teams performed experiments that produced results relating to sample homogeneity. RESULTS: The data, which were produced via two different approaches (a radiolabeled and a nonradiolabeled approach), are highly complementary and demonstrate clear effects on sample inhomogeneity due to the substrate type, compound and hematocrit levels. CONCLUSION: The results demonstrate that sample inhomogeneity is a significant hurdle to the use of dried blood spots for regulated bioanalysis that should be investigated further in the method establishment phase if the whole spot is not sampled.
Authors: Helena Beatriz Ferreira; Inês M S Guerra; Tânia Melo; Hugo Rocha; Ana S P Moreira; Artur Paiva; M Rosário Domingues Journal: Anal Bioanal Chem Date: 2022-07-15 Impact factor: 4.478
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Authors: Kayla N Ellefsen; Jose Luiz da Costa; Marta Concheiro; Sebastien Anizan; Allan J Barnes; Sandrine Pirard; David A Gorelick; Marilyn A Huestis Journal: Bioanalysis Date: 2015 Impact factor: 2.681
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