Literature DB >> 26319592

A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG.

G M Proshkina1, O N Shilova1, A V Ryabova2, O A Stremovskiy1, S M Deyev3.   

Abstract

Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
Copyright © 2015 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Entities:  

Keywords:  Blue light illumination; DARPin; HER2/neu; miniSOG

Mesh：

Substances：

Year:  2015        PMID： 26319592     DOI： 10.1016/j.biochi.2015.08.013

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  12 in total

1.  The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor Internalization.

Authors:  E O Kuzichkina; O N Shilova; S M Deyev; R V Petrov
Journal:  Dokl Biochem Biophys       Date:  2018-11-05       Impact factor: 0.788

2.  Radioactive (90Y) upconversion nanoparticles conjugated with recombinant targeted toxin for synergistic nanotheranostics of cancer.

Authors:  Evgenii L Guryev; Natalia O Volodina; Natalia Y Shilyagina; Sergey V Gudkov; Irina V Balalaeva; Arthur B Volovetskiy; Alexander V Lyubeshkin; Alexey V Sen'; Sergey A Ermilov; Vladimir A Vodeneev; Rem V Petrov; Andrei V Zvyagin; Zhores I Alferov; Sergey M Deyev
Journal:  Proc Natl Acad Sci U S A       Date:  2018-09-07       Impact factor: 11.205

3.  Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization.

Authors:  E I Shramova; G M Proshkina; S M Deyev; R V Petrov
Journal:  Dokl Biochem Biophys       Date:  2017-07-20       Impact factor: 0.788

Review 4.  Blue-Light Receptors for Optogenetics.

Authors:  Aba Losi; Kevin H Gardner; Andreas Möglich
Journal:  Chem Rev       Date:  2018-07-09       Impact factor: 60.622

5.  Cytotoxicity of targeted HER2-specific phototoxins based on flavoprotein miniSOG is determined by the rate of their internalization.

Authors:  O N Shilova; G M Proshkina; A V Ryabova; S M Deyev; R V Petrov
Journal:  Dokl Biochem Biophys       Date:  2017-09-02       Impact factor: 0.788

6.  Bioreactor-Based Tumor Tissue Engineering.

Authors:  A E Guller; P N Grebenyuk; A B Shekhter; A V Zvyagin; S M Deyev
Journal:  Acta Naturae       Date:  2016 Jul-Sep       Impact factor: 1.845

7.  Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer.

Authors:  E I Shramova; G M Proshkina; S P Chumakov; Yu M Khodarovich; S M Deyev
Journal:  Acta Naturae       Date:  2016 Oct-Dec       Impact factor: 1.845

8.  Penetration Efficiency of Antitumor Agents in Ovarian Cancer Spheroids: The Case of Recombinant Targeted Toxin DARPin-LoPE and the Chemotherapy Drug, Doxorubicin.

Authors:  Evgeniya Sokolova; Olga Kutova; Alena Grishina; Anton Pospelov; Evgeniy Guryev; Alexey Schulga; Sergey Deyev; Irina Balalaeva
Journal:  Pharmaceutics       Date:  2019-05-07       Impact factor: 6.321

Review 9.  Natural and Designed Toxins for Precise Therapy: Modern Approaches in Experimental Oncology.

Authors:  Olga Shilova; Elena Shramova; Galina Proshkina; Sergey Deyev
Journal:  Int J Mol Sci       Date:  2021-05-07       Impact factor: 5.923

10.  The Effect of the Targeted Recombinant Toxin DARPin-PE40 on the Dynamics of HER2-Positive Tumor Growth.

Authors:  E A Sokolova; G M Proshkina; O M Kutova; I V Balalaeva; S M Deyev
Journal:  Acta Naturae       Date:  2017 Jul-Sep       Impact factor: 1.845
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