Literature DB >> 30397884

The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor Internalization.

E O Kuzichkina1,2, O N Shilova3, S M Deyev3, R V Petrov3.   

Abstract

It was found that the fluorescent properties of the phototoxic domain of miniSOG allow to assess the ability of toxins to bind to human breast adenocarcinoma cells SK-BR-3 and study the dynamics of their internalization. We established that the main cause of the decrease of the fluorescence intensity of the recombinant proteins 4D5scFv-miniSOG and DARPin-miniSOG during their internalization in the complex with the HER2 receptor is their shielding and absorption of the fluorescence of miniSOG by the cells fluorophores.

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Year:  2018        PMID： 30397884     DOI： 10.1134/S1607672918050046

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  10 in total

1.  A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG.

Authors:  G M Proshkina; O N Shilova; A V Ryabova; O A Stremovskiy; S M Deyev
Journal:  Biochimie       Date:  2015-08-28       Impact factor: 4.079

2.  Method for real-time monitoring of protein degradation at the single cell level.

Authors:  Lijuan Zhang; Nadya G Gurskaya; Ekaterina M Merzlyak; Dmitry B Staroverov; Nikolay N Mudrik; Olga N Samarkina; Leonid M Vinokurov; Sergey Lukyanov; Konstantin A Lukyanov
Journal:  Biotechniques       Date:  2007-04       Impact factor: 1.993

3.  Cellular autofluorescence--is it due to flavins?

Authors:  R C Benson; R A Meyer; M E Zaruba; G M McKhann
Journal:  J Histochem Cytochem       Date:  1979-01       Impact factor: 2.479

4.  The effect of trypan blue treatment on autofluorescence of fixed cells.

Authors:  Olga N Shilova; Evgeny S Shilov; Sergey M Deyev
Journal:  Cytometry A       Date:  2017-08-30       Impact factor: 4.355

Review 5.  Memory Retrieval in Mice and Men.

Authors:  Aya Ben-Yakov; Yadin Dudai; Mark R Mayford
Journal:  Cold Spring Harb Perspect Biol       Date:  2015-10-05       Impact factor: 10.005

6.  Cytotoxicity of targeted HER2-specific phototoxins based on flavoprotein miniSOG is determined by the rate of their internalization.

Authors:  O N Shilova; G M Proshkina; A V Ryabova; S M Deyev; R V Petrov
Journal:  Dokl Biochem Biophys       Date:  2017-09-02       Impact factor: 0.788

7.  Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene.

Authors:  D J Slamon; G M Clark; S G Wong; W J Levin; A Ullrich; W L McGuire
Journal:  Science       Date:  1987-01-09       Impact factor: 47.728

Review 8.  Endocytosis and intracellular trafficking of ErbBs.

Authors:  Alexander Sorkin; Lai Kuan Goh
Journal:  Exp Cell Res       Date:  2009-02-15       Impact factor: 3.905

9.  pH in the endosome. Measurements during pinocytosis and receptor-mediated endocytosis.

Authors:  M J Geisow; W H Evans
Journal:  Exp Cell Res       Date:  1984-01       Impact factor: 3.905

10.  Genetically encoded immunophotosensitizer 4D5scFv-miniSOG is a highly selective agent for targeted photokilling of tumor cells in vitro.

Authors:  Kristina E Mironova; Galina M Proshkina; Anastasiya V Ryabova; Oleg A Stremovskiy; Sergey A Lukyanov; Rem V Petrov; Sergey M Deyev
Journal:  Theranostics       Date:  2013-10-12       Impact factor: 11.556
  10 in total
  2 in total

Review 1.  A Photosensitized Singlet Oxygen (1O2) Toolbox for Bio-Organic Applications: Tailoring 1O2 Generation for DNA and Protein Labelling, Targeting and Biosensing.

Authors:  Dorien Aerssens; Enrico Cadoni; Laure Tack; Annemieke Madder
Journal:  Molecules       Date:  2022-01-25       Impact factor: 4.411

2.  Multimerization through Pegylation Improves Pharmacokinetic Properties of scFv Fragments of GD2-Specific Antibodies.

Authors:  Irina V Kholodenko; Daniel V Kalinovsky; Elena V Svirshchevskaya; Igor I Doronin; Maria V Konovalova; Alexey V Kibardin; Tatyana V Shamanskaya; Sergey S Larin; Sergey M Deyev; Roman V Kholodenko
Journal:  Molecules       Date:  2019-10-24       Impact factor: 4.411
  2 in total

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