Teresa Conceição1, Céline Coelho1, Hermínia de Lencastre2, Marta Aires-de-Sousa3. 1. Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal. 2. Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, NY, USA. 3. Escola Superior de Saúde da Cruz Vermelha Portuguesa (ESSCVP), Lisboa, Portugal msousa@esscvp.eu.
Abstract
BACKGROUND: Oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) isolates have been increasingly reported worldwide, but data regarding the African continent have not been available. METHODS: Between 2010 and 2014, 1462 inpatients and healthcare workers were screened for MRSA nasal carriage in São Tomé and Príncipe (STP) and Angola, two Portuguese-speaking African countries (PALOP countries). We determined the presence of the mecA gene and the antimicrobial susceptibility profiles of the isolates. OS-MRSA clonal lineages were identified as well as the presence of virulence determinants, including Panton-Valentine leucocidin (PVL). RESULTS: Out of 164 S. aureus hospital isolates tested, 29 (17.7%) were mecA positive, but susceptible to oxacillin, showing oxacillin MICs ≤3 mg/L. All OS-MRSA isolates were resistant to cefoxitin and most of them were also resistant to at least two antimicrobials other than β-lactams. The 29 OS-MRSA were distributed into two major clonal lineages: (i) PFGE type B-ST88-SCCmec IVa, associated with spa types t186/t325/t786/t1814/t1951, detected in Angola (n = 5) and STP (n = 10); and (ii) PFGE type C-t451/t648-ST8-SCCmec V, exclusively found in STP (n = 9). OS-MRSA showed at least two virulence determinants. PVL was detected in an isolate recovered in STP. CONCLUSIONS: We describe a high prevalence of OS-MRSA among S. aureus strains recovered in two African countries. OS-MRSA in PALOP countries were mainly associated with ST88 and ST8, two prevalent MRSA clonal types in these countries. If direct testing for mecA is not available, cefoxitin susceptibility testing is highly recommended to avoid the misidentification of OS-MRSA.
BACKGROUND:Oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) isolates have been increasingly reported worldwide, but data regarding the African continent have not been available. METHODS: Between 2010 and 2014, 1462 inpatients and healthcare workers were screened for MRSA nasal carriage in São Tomé and Príncipe (STP) and Angola, two Portuguese-speaking African countries (PALOP countries). We determined the presence of the mecA gene and the antimicrobial susceptibility profiles of the isolates. OS-MRSA clonal lineages were identified as well as the presence of virulence determinants, including Panton-Valentine leucocidin (PVL). RESULTS: Out of 164 S. aureus hospital isolates tested, 29 (17.7%) were mecA positive, but susceptible to oxacillin, showing oxacillin MICs ≤3 mg/L. All OS-MRSA isolates were resistant to cefoxitin and most of them were also resistant to at least two antimicrobials other than β-lactams. The 29 OS-MRSA were distributed into two major clonal lineages: (i) PFGE type B-ST88-SCCmec IVa, associated with spa types t186/t325/t786/t1814/t1951, detected in Angola (n = 5) and STP (n = 10); and (ii) PFGE type C-t451/t648-ST8-SCCmec V, exclusively found in STP (n = 9). OS-MRSA showed at least two virulence determinants. PVL was detected in an isolate recovered in STP. CONCLUSIONS: We describe a high prevalence of OS-MRSA among S. aureus strains recovered in two African countries. OS-MRSA in PALOP countries were mainly associated with ST88 and ST8, two prevalent MRSA clonal types in these countries. If direct testing for mecA is not available, cefoxitin susceptibility testing is highly recommended to avoid the misidentification of OS-MRSA.
Authors: Marilyn Chung; Choon Keun Kim; Teresa Conceição; Marta Aires-De-Sousa; Hermínia De Lencastre; Alexander Tomasz Journal: J Antimicrob Chemother Date: 2016-06-07 Impact factor: 5.790
Authors: K V Okuda; N Toepfner; A S Alabi; B Arnold; S Bélard; U Falke; L Menschner; S Monecke; A Ruppelt-Lorz; R Berner Journal: Eur J Clin Microbiol Infect Dis Date: 2016-08-23 Impact factor: 5.103
Authors: Magdalena Wysocka; Tamar Monteiro; Carine de Pina; Deisy Gonçalves; Sandrine de Pina; Antonio Ludgero-Correia; Joao Moreno; Roxana Zamudio; Nada Almebairik; Laura J Gray; Manish Pareek; David R Jenkins; Marta Aires-de-Sousa; Herminia De Lencastre; Sandra Beleza; Isabel I Araujo; Teresa Conceição; Marco R Oggioni Journal: J Glob Antimicrob Resist Date: 2021-05-27 Impact factor: 4.035
Authors: Wenjing Geng; Yujie Qi; Wenting Li; Thomas H McConville; Alexandra Hill-Ricciuti; Emily C Grohs; Lisa Saiman; Anne-Catrin Uhlemann Journal: PLoS One Date: 2020-02-13 Impact factor: 3.240