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4 in total

1. Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

Authors: V C Sheffield; D R Cox; L S Lerman; R M Myers
Journal: Proc Natl Acad Sci U S A Date: 1989-01 Impact factor: 11.205

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

3. The locus of sequence-directed and protein-induced DNA bending.

Authors: H M Wu; D M Crothers
Journal: Nature Date: 1984 Apr 5-11 Impact factor: 49.962

4. Chromosome mapping and expression of a putative testis-determining gene in mouse.

Authors: C M Nagamine; K M Chan; C A Kozak; Y F Lau
Journal: Science Date: 1989-01-06 Impact factor: 47.728

4 in total

46 in total

1. Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences.

Authors: A G Speksnijder; G A Kowalchuk; S De Jong; E Kline; J R Stephen; H J Laanbroek
Journal: Appl Environ Microbiol Date: 2001-01 Impact factor: 4.792

2. Combined SSCP/duplex analysis by capillary electrophoresis for more efficient mutation detection.

Authors: P Kozlowski; W J Krzyzosiak
Journal: Nucleic Acids Res Date: 2001-07-15 Impact factor: 16.971

3. In vitro gene amplification for prenatal diagnosis of congenital adrenal hyperplasia.

Authors: G Rumsby; J W Honour
Journal: J Med Genet Date: 1990-11 Impact factor: 6.318

4. The molecular defect in a family with mild atypical osteogenesis imperfecta and extreme joint hypermobility: exon skipping caused by an 11-bp deletion from an intron in one COL1A2 allele.

Authors: A C Nicholls; J Oliver; D V Renouf; D A Heath; F M Pope
Journal: Hum Genet Date: 1992-03 Impact factor: 4.132

10. Use of the single-strand conformational polymorphism method to detect recurrent and novel mutations in the low-density lipoprotein receptor gene in patients with familial hypercholesterolaemia: detection of a novel mutation Asp200-->Gly.

Authors: V Gudnason; Y T Mak; J Betteridge; S N McCarthy; S Humphries
Journal: Clin Investig Date: 1993-04

A PCR artifact: generation of heteroduplexes.

1. Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

3. The locus of sequence-directed and protein-induced DNA bending.

4. Chromosome mapping and expression of a putative testis-determining gene in mouse.

1. Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences.

2. Combined SSCP/duplex analysis by capillary electrophoresis for more efficient mutation detection.

3. In vitro gene amplification for prenatal diagnosis of congenital adrenal hyperplasia.

4. The molecular defect in a family with mild atypical osteogenesis imperfecta and extreme joint hypermobility: exon skipping caused by an 11-bp deletion from an intron in one COL1A2 allele.

5. Elimination of heteroduplex artifacts when sequencing HLA genes amplified by polymerase chain reaction (PCR).

6. A rare mutation in the primer binding region of the amelogenin gene can interfere with gender identification.

7. Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism.

8. Heteroduplex formation as a means to exclude contamination in virus detection using PCR.

9. Diagnostic heteroduplexes: simple detection of carriers of a 4-bp insertion mutation in Tay-Sachs disease.

10. Use of the single-strand conformational polymorphism method to detect recurrent and novel mutations in the low-density lipoprotein receptor gene in patients with familial hypercholesterolaemia: detection of a novel mutation Asp200-->Gly.