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Literature DB >> 11133483

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences.

A G Speksnijder¹, G A Kowalchuk, S De Jong, E Kline, J R Stephen, H J Laanbroek.

Abstract

A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone libraries.

Mesh：

Substances：
RNA, Ribosomal, 16S

Year: 2001 PMID： 11133483 PMCID： PMC92603 DOI： 10.1128/AEM.67.1.469-472.2001

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

25 in total

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Authors: A G Speksnijder; G A Kowalchuk; K Roest; H J Laanbroek
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7. Biodiversity, community structural shifts, and biogeography of prokaryotes within Antarctic continental shelf sediment.

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8. Combining culture-dependent and -independent methodologies for estimation of richness of estuarine bacterioplankton consuming riverine dissolved organic matter.

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9. Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach.

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