Literature DB >> 26269182

Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication.

Yongqian Zhao1, Tingjin Sherryl Soh2, Kitti Wing Ki Chan3, Sarah Suet Yin Fung3, Kunchithapadam Swaminathan4, Siew Pheng Lim5, Pei-Yong Shi5, Thomas Huber6, Julien Lescar7, Dahai Luo8, Subhash G Vasudevan9.   

Abstract

We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In contrast, Pro substitution abolished virus replication by imposing rigidity in the linker and restricting NS5's conformational plasticity. Our biochemical and reverse genetics experiments demonstrate that NS5 utilizes conformational regulation to achieve optimum viral replication.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26269182      PMCID： PMC4580200          DOI： 10.1128/JVI.01239-15

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  23 in total

1.  Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution.

Authors:  Thai Leong Yap; Ting Xu; Yen-Liang Chen; Helene Malet; Marie-Pierre Egloff; Bruno Canard; Subhash G Vasudevan; Julien Lescar
Journal:  J Virol       Date:  2007-02-14       Impact factor: 5.103

2.  Inhibition of dengue virus polymerase by blocking of the RNA tunnel.

Authors:  Pornwaratt Niyomrattanakit; Yen-Liang Chen; Hongping Dong; Zheng Yin; Min Qing; J Frasier Glickman; Kai Lin; Dieter Mueller; Hans Voshol; Joanne Y H Lim; Shahul Nilar; Thomas H Keller; Pei-Yong Shi
Journal:  J Virol       Date:  2010-03-17       Impact factor: 5.103

Review 3.  Ten years of dengue drug discovery: progress and prospects.

Authors:  Siew Pheng Lim; Qing-Yin Wang; Christian G Noble; Yen-Liang Chen; Hongping Dong; Bin Zou; Fumiaki Yokokawa; Shahul Nilar; Paul Smith; David Beer; Julien Lescar; Pei-Yong Shi
Journal:  Antiviral Res       Date:  2013-09-27       Impact factor: 5.970

4.  Small molecule inhibitors that selectively block dengue virus methyltransferase.

Authors:  Siew Pheng Lim; Louis Sebastian Sonntag; Christian Noble; Shahul H Nilar; Ru Hui Ng; Gang Zou; Paul Monaghan; Ka Yan Chung; Hongping Dong; Boping Liu; Christophe Bodenreider; Gladys Lee; Mei Ding; Wai Ling Chan; Gang Wang; Yap Li Jian; Alexander Theodore Chao; Julien Lescar; Zheng Yin; T R Vedananda; Thomas H Keller; Pei-Yong Shi
Journal:  J Biol Chem       Date:  2010-12-08       Impact factor: 5.157

5.  Structure and function of flavivirus NS5 methyltransferase.

Authors:  Yangsheng Zhou; Debashish Ray; Yiwei Zhao; Hongping Dong; Suping Ren; Zhong Li; Yi Guo; Kristen A Bernard; Pei-Yong Shi; Hongmin Li
Journal:  J Virol       Date:  2007-01-31       Impact factor: 5.103

6.  Dengue virus nonstructural protein 5 adopts multiple conformations in solution.

Authors:  Cécile Bussetta; Kyung H Choi
Journal:  Biochemistry       Date:  2012-07-16       Impact factor: 3.162

7.  West Nile virus methyltransferase catalyzes two methylations of the viral RNA cap through a substrate-repositioning mechanism.

Authors:  Hongping Dong; Suping Ren; Bo Zhang; Yangsheng Zhou; Francesc Puig-Basagoiti; Hongmin Li; Pei-Yong Shi
Journal:  J Virol       Date:  2008-02-27       Impact factor: 5.103

8.  Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface.

Authors:  Guoliang Lu; Peng Gong
Journal:  PLoS Pathog       Date:  2013-08-08       Impact factor: 6.823

9.  A crystal structure of the dengue virus non-structural protein 5 (NS5) polymerase delineates interdomain amino acid residues that enhance its thermostability and de novo initiation activities.

Authors:  Siew Pheng Lim; Jolene Hong Kiew Koh; Cheah Chen Seh; Chong Wai Liew; Andrew D Davidson; Leng Shiew Chua; Ramya Chandrasekaran; Tobias C Cornvik; Pei-Yong Shi; Julien Lescar
Journal:  J Biol Chem       Date:  2013-09-11       Impact factor: 5.157

10.  RNA-dependent RNA polymerase of Japanese encephalitis virus binds the initiator nucleotide GTP to form a mechanistically important pre-initiation state.

Authors:  Parag Surana; Vijaya Satchidanandam; Deepak T Nair
Journal:  Nucleic Acids Res       Date:  2013-11-28       Impact factor: 16.971
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  20 in total

Review 1.  The Many Faces of the Flavivirus NS5 Protein in Antagonism of Type I Interferon Signaling.

Authors:  Sonja M Best
Journal:  J Virol       Date:  2017-01-18       Impact factor: 5.103

2.  Two RNA Tunnel Inhibitors Bind in Highly Conserved Sites in Dengue Virus NS5 Polymerase: Structural and Functional Studies.

Authors:  Rishi Arora; Chong Wai Liew; Tingjin Sherryl Soh; Dorcas Adobea Otoo; Cheah Chen Seh; Kimberley Yue; Shahul Nilar; Gang Wang; Fumiaki Yokokawa; Christian G Noble; Yen Liang Chen; Pei-Yong Shi; Julien Lescar; Thomas M Smith; Timothy E Benson; Siew Pheng Lim
Journal:  J Virol       Date:  2020-11-23       Impact factor: 5.103

3.  Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin.

Authors:  Ankita Pan; Wuan Geok Saw; Malathy Sony Subramanian Manimekalai; Ardina Grüber; Shin Joon; Tsutomu Matsui; Thomas M Weiss; Gerhard Grüber
Journal:  Acta Crystallogr D Struct Biol       Date:  2017-04-19       Impact factor: 7.652

4.  In silico targeting of non-structural 4B protein from dengue virus 4 with spiropyrazolopyridone: study of molecular dynamics simulation, ADMET and virtual screening.

Authors:  Waqar Hussain; Iqra Qaddir; Sajid Mahmood; Nouman Rasool
Journal:  Virusdisease       Date:  2018-03-29

5.  NS5 from Dengue Virus Serotype 2 Can Adopt a Conformation Analogous to That of Its Zika Virus and Japanese Encephalitis Virus Homologues.

Authors:  Abbas El Sahili; Tingjin Sherryl Soh; Jonas Schiltz; Aïcha Gharbi-Ayachi; Cheah Chen Seh; Pei-Yong Shi; Siew Pheng Lim; Julien Lescar
Journal:  J Virol       Date:  2019-12-12       Impact factor: 5.103

6.  Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives.

Authors:  Bruno Coutard; Karine Barral; Julie Lichière; Barbara Selisko; Baptiste Martin; Wahiba Aouadi; Miguel Ortiz Lombardia; Françoise Debart; Jean-Jacques Vasseur; Jean Claude Guillemot; Bruno Canard; Etienne Decroly
Journal:  J Virol       Date:  2017-02-14       Impact factor: 5.103

7.  Serotype-specific interactions among functional domains of dengue virus 2 nonstructural proteins (NS) 5 and NS3 are crucial for viral RNA replication.

Authors:  Tadahisa Teramoto; Anuradha Balasubramanian; Kyung H Choi; Radhakrishnan Padmanabhan
Journal:  J Biol Chem       Date:  2017-04-10       Impact factor: 5.157

8.  Molecular basis for specific viral RNA recognition and 2'-O-ribose methylation by the dengue virus nonstructural protein 5 (NS5).

Authors:  Yongqian Zhao; Tingjin Sherryl Soh; Siew Pheng Lim; Ka Yan Chung; Kunchithapadam Swaminathan; Subhash G Vasudevan; Pei-Yong Shi; Julien Lescar; Dahai Luo
Journal:  Proc Natl Acad Sci U S A       Date:  2015-11-17       Impact factor: 11.205

9.  Amino Acid Substitutions in NS5 Contribute Differentially to Tembusu Virus Attenuation in Ducklings and Cell Cultures.

Authors:  Xue Sun; Mengxu Sun; Lijiao Zhang; Ziding Yu; Jinxin Li; Wanying Xie; Jingliang Su
Journal:  Viruses       Date:  2021-05-16       Impact factor: 5.048

Review 10.  Molecular Insights into the Flavivirus Replication Complex.

Authors:  Kaïn van den Elsen; Jun Ping Quek; Dahai Luo
Journal:  Viruses       Date:  2021-05-21       Impact factor: 5.048
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