Literature DB >> 26266832

Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.

Enrico Ravera1, Vladimir K Michaelis2, Ta-Chung Ong2,3, Eric G Keeler2, Tommaso Martelli1,4, Marco Fragai1, Robert G Griffin2, Claudio Luchinat1.   

Abstract

Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for long-term storage and reusability. Recently, it was shown that an atomic-level description of proteins immobilized in a biosilica matrix can be attained by examining their magic-angle spinning (MAS) NMR spectra. However, even though MAS NMR is an excellent tool for determining structure, it is severely hampered by sensitivity. In this work we provide the proof of principle that NMR characterization of biosilica-entrapped enzymes could be assisted by high-field dynamic nuclear polarization (DNP).
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  DNP; biomaterials; biosilica; enzyme immobilization; solid-state NMR

Year:  2015        PMID： 26266832      PMCID： PMC4752418          DOI： 10.1002/cphc.201500549

Source DB:  PubMed          Journal:  Chemphyschem        ISSN： 1439-4235            Impact factor:   3.102


  28 in total

1.  Biosilica formation in diatoms: characterization of native silaffin-2 and its role in silica morphogenesis.

Authors:  Nicole Poulsen; Manfred Sumper; Nils Kröger
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-24       Impact factor: 11.205

2.  Surface enhanced NMR spectroscopy by dynamic nuclear polarization.

Authors:  Anne Lesage; Moreno Lelli; David Gajan; Marc A Caporini; Veronika Vitzthum; Pascal Miéville; Johan Alauzun; Arthur Roussey; Chloé Thieuleux; Ahmad Mehdi; Geoffrey Bodenhausen; Christophe Copéret; Lyndon Emsley
Journal:  J Am Chem Soc       Date:  2010-11-10       Impact factor: 15.419

3.  Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution.

Authors:  D A Hall; D C Maus; G J Gerfen; S J Inati; L R Becerra; F W Dahlquist; R G Griffin
Journal:  Science       Date:  1997-05-09       Impact factor: 47.728

4.  Topical Developments in High-Field Dynamic Nuclear Polarization.

Authors:  Vladimir K Michaelis; Ta-Chung Ong; Matthew K Kiesewetter; Derik K Frantz; Joseph J Walish; Enrico Ravera; Claudio Luchinat; Timothy M Swager; Robert G Griffin
Journal:  Isr J Chem       Date:  2014-02-13       Impact factor: 3.333

5.  TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media.

Authors:  Changsik Song; Kan-Nian Hu; Chan-Gyu Joo; Timothy M Swager; Robert G Griffin
Journal:  J Am Chem Soc       Date:  2006-09-06       Impact factor: 15.419

6.  Quantum mechanical theory of dynamic nuclear polarization in solid dielectrics.

Authors:  Kan-Nian Hu; Galia T Debelouchina; Albert A Smith; Robert G Griffin
Journal:  J Chem Phys       Date:  2011-03-28       Impact factor: 3.488

7.  Solvent-free dynamic nuclear polarization of amorphous and crystalline ortho-terphenyl.

Authors:  Ta-Chung Ong; Melody L Mak-Jurkauskas; Joseph J Walish; Vladimir K Michaelis; Björn Corzilius; Albert A Smith; Andrew M Clausen; Janet C Cheetham; Timothy M Swager; Robert G Griffin
Journal:  J Phys Chem B       Date:  2013-03-04       Impact factor: 2.991

8.  Dynamic nuclear polarization of sedimented solutes.

Authors:  Enrico Ravera; Björn Corzilius; Vladimir K Michaelis; Camilla Rosa; Robert G Griffin; Claudio Luchinat; Ivano Bertini
Journal:  J Am Chem Soc       Date:  2013-01-25       Impact factor: 15.419

9.  High-resolution solid-state NMR structure of a 17.6 kDa protein.

Authors:  Ivano Bertini; Anusarka Bhaumik; Gaël De Paëpe; Robert G Griffin; Moreno Lelli; Józef R Lewandowski; Claudio Luchinat
Journal:  J Am Chem Soc       Date:  2010-01-27       Impact factor: 15.419

10.  High field dynamic nuclear polarization NMR with surfactant sheltered biradicals.

Authors:  Matthew K Kiesewetter; Vladimir K Michaelis; Joseph J Walish; Robert G Griffin; Timothy M Swager
Journal:  J Phys Chem B       Date:  2014-02-07       Impact factor: 2.991
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  5 in total

1.  Ubiquitin immobilized on mesoporous MCM41 silica surfaces - Analysis by solid-state NMR with biophysical and surface characterization.

Authors:  Nurit Adiram-Filiba; Avital Schremer; Eli Ohaion; Merav Nadav-Tsubery; Tammi Lublin-Tennenbaum; Keren Keinan-Adamsky; Gil Goobes
Journal:  Biointerphases       Date:  2017-05-31       Impact factor: 2.456

2.  19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies.

Authors:  Manman Lu; Mingzhang Wang; Ivan V Sergeyev; Caitlin M Quinn; Jochem Struppe; Melanie Rosay; Werner Maas; Angela M Gronenborn; Tatyana Polenova
Journal:  J Am Chem Soc       Date:  2019-04-01       Impact factor: 15.419

3.  Lysozyme is Sterically Trapped Within the Silica Cage in Bioinspired Silica-Lysozyme Composites: A Multi-Technique Understanding of Elusive Protein-Material Interactions.

Authors:  Francesco Bruno; Lucia Gigli; Giovanni Ferraro; Andrea Cavallo; Vladimir K Michaelis; Gil Goobes; Emiliano Fratini; Enrico Ravera
Journal:  Langmuir       Date:  2022-06-23       Impact factor: 4.331

4.  (1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization.

Authors:  Enrico Ravera; Linda Cerofolini; Tommaso Martelli; Alexandra Louka; Marco Fragai; Claudio Luchinat
Journal:  Sci Rep       Date:  2016-06-09       Impact factor: 4.379

5.  On the Mechanism of Bioinspired Formation of Inorganic Oxides: Structural Evidence of the Electrostatic Nature of the Interaction between a Mononuclear Inorganic Precursor and Lysozyme.

Authors:  Lucia Gigli; Enrico Ravera; Vito Calderone; Claudio Luchinat
Journal:  Biomolecules       Date:  2020-12-30
  5 in total

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