| Literature DB >> 26256325 |
Xuejing Jia1, Chao Zhang1, Jianfeng Qiu1, Lili Wang2, Jiaolin Bao1, Kai Wang1, Yulin Zhang1, Meiwan Chen1, Jianbo Wan1, Huanxing Su1, Jianping Han2, Chengwei He3.
Abstract
Three novel acidic polysaccharides termed PRM1, PRM3 and PRM5 were purified from Rhynchosia minima root using DEAE-52 cellulose and sephadex G-150 column chromatography. Their structures were characterized by ultraviolet (UV) and Fourier transform infrared (FTIR) spectrometry, gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), and differential scanning colorimeter (DSC) analysis. The uronic acid contents of PRM1, PRM3 and PRM5 were 30.7%, 12.7% and 47.7%, respectively. PRM1 (143.2 kDa), PRM3 (105.3 kDa) and PRM5 (162.1 kDa) were heteropolysaccharides because they were composed of arabinose, mannose, glucose and galactose. Their enthalpy values were 201.0, 111.0 and 206.8 J/g, respectively. PRM3 and PRM1 exhibited strong in vitro anticancer activity against lung cancer A549 and liver cancer HepG2 cells in a dose-dependent manner. These findings suggested that PRM1 and PRM3 could be potentially developed as natural anticancer agents.Entities:
Keywords: Anticancer; Polysaccharide; Purification; Rhynchosia minima
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Year: 2015 PMID： 26256325 DOI： 10.1016/j.carbpol.2015.05.059
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381