Yong Guan1, Shuangde Liu2, Yuqiang Liu1, Chao Sun3, Guanghui Cheng3, Yun Luan3, Kailin Li3, Jue Wang3, Xiaoshuai Xie1, Shengtian Zhao4. 1. Department of Urology, The Second Hospital, Shandong University, China. 2. Department of Kidney Transplantation, The Second Hospital, Shandong University, China. 3. Department of Central Research Laboratory, The Second Hospital, Shandong University, China. 4. Department of Urology, The Second Hospital, Shandong University, China. Electronic address: zhaoshengtian@sdu.edu.cn.
Abstract
OBJECTIVE: To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research. METHODS: Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization. RESULTS: Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components. CONCLUSION: We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques.
OBJECTIVE: To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research. METHODS: Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization. RESULTS: Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components. CONCLUSION: We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques.
Authors: L C P C Leonel; C M F C Miranda; T M Coelho; G A S Ferreira; R R Caãada; M A Miglino; S E Lobo Journal: Braz J Med Biol Res Date: 2017-11-17 Impact factor: 2.590
Authors: Stacey M S Gruber; Paulomi Ghosh; Karl Wilhelm Mueller; Patrick W Whitlock; Chia-Ying Lin Journal: J Vis Exp Date: 2019-01-07 Impact factor: 1.355