Panagiotis Mallis1, Charalampos Oikonomidis2, Zetta Dimou2, Catherine Stavropoulos-Giokas2, Efstathios Michalopoulos2, Michalis Katsimpoulas3. 1. Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27, Athens, Greece. pmallis@bioacademy.gr. 2. Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27, Athens, Greece. 3. Center of Experimental Surgery, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27, Athens, Greece.
Abstract
BACKGROUND: Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing mostly the tissue engineering approaches. Unlike to perfusion decellularization of kidneys, we proposed alternative decellularization strategies to obtain acellular kidney scaffolds. The aim of this study was the evaluation of two different decellularization approaches for producing kidney bioscaffolds. METHODS: Rat kidneys from Wistar rats, were submitted to decellularization, followed two different strategies. The decellularization solutions used in both approaches were the same and involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate and sodium dodecyl sulfate buffers for 12 h each, followed by incubation in a serum medium. Both approaches involved 3 decellularization cycles. Histological analysis, biochemical and DNA quantification were performed. Cytotoxicity assay and repopulation of acellular kidneys were also applied. RESULTS: Histological, biochemical and DNA quantification confirmed that the 2nd approach had the best outcome regarding the kidney composition and cell elimination. Acellular kidneys from both approaches were successfully recellularized. CONCLUSION: Based on the above data, the production of kidney scaffolds with the proposed cost- effective decellularization approaches, was efficient.
BACKGROUND: Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing mostly the tissue engineering approaches. Unlike to perfusion decellularization of kidneys, we proposed alternative decellularization strategies to obtain acellular kidney scaffolds. The aim of this study was the evaluation of two different decellularization approaches for producing kidney bioscaffolds. METHODS: Rat kidneys from Wistar rats, were submitted to decellularization, followed two different strategies. The decellularization solutions used in both approaches were the same and involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate and sodium dodecyl sulfate buffers for 12 h each, followed by incubation in a serum medium. Both approaches involved 3 decellularization cycles. Histological analysis, biochemical and DNA quantification were performed. Cytotoxicity assay and repopulation of acellular kidneys were also applied. RESULTS: Histological, biochemical and DNA quantification confirmed that the 2nd approach had the best outcome regarding the kidney composition and cell elimination. Acellular kidneys from both approaches were successfully recellularized. CONCLUSION: Based on the above data, the production of kidney scaffolds with the proposed cost- effective decellularization approaches, was efficient.
Authors: Yimin Lu; Christina Stamm; Dina Nobre; Menno Pruijm; Daniel Teta; Anne Cherpillod; Georges Halabi; Olivier Phan; Zina Fumeaux; Roberto Bullani; Thierry Gauthier; Claudine Mathieu; Michel Burnier; Anne Zanchi Journal: Swiss Med Wkly Date: 2017-07-06 Impact factor: 2.193
Authors: Andrew S Levey; Kai-Uwe Eckardt; Nijsje M Dorman; Stacy L Christiansen; Ewout J Hoorn; Julie R Ingelfinger; Lesley A Inker; Adeera Levin; Rajnish Mehrotra; Paul M Palevsky; Mark A Perazella; Allison Tong; Susan J Allison; Detlef Bockenhauer; Josephine P Briggs; Jonathan S Bromberg; Andrew Davenport; Harold I Feldman; Denis Fouque; Ron T Gansevoort; John S Gill; Eddie L Greene; Brenda R Hemmelgarn; Matthias Kretzler; Mark Lambie; Pascale H Lane; Joseph Laycock; Shari E Leventhal; Michael Mittelman; Patricia Morrissey; Marlies Ostermann; Lesley Rees; Pierre Ronco; Franz Schaefer; Jennifer St Clair Russell; Caroline Vinck; Stephen B Walsh; Daniel E Weiner; Michael Cheung; Michel Jadoul; Wolfgang C Winkelmayer Journal: Kidney Int Date: 2020-03-09 Impact factor: 10.612
Authors: Benjamin Bowe; Yan Xie; Tingting Li; Ali H Mokdad; Hong Xian; Yan Yan; Geetha Maddukuri; Ziyad Al-Aly Journal: JAMA Netw Open Date: 2018-11-02