| Literature DB >> 26193423 |
Mercedes C Hernandez-Gomez1, Maja G Rydahl2, Artur Rogowski3, Carl Morland3, Alan Cartmell3, Lucy Crouch3, Aurore Labourel3, Carlos M G A Fontes4, William G T Willats2, Harry J Gilbert5, J Paul Knox6.
Abstract
Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3acipA, are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β-1,4-glucan containing α-1,6-xylose side chains. CtCBM3acipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3acipA. A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed.Entities:
Keywords: CBM3a; Carbohydrate-binding module; Crystalline cellulose; Plant cell wall; Xyloglucan
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Year: 2015 PMID： 26193423 PMCID： PMC5877785 DOI： 10.1016/j.febslet.2015.07.009
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124