Ananda Malta1, Egberto Gaspar de Moura2, Tatiane Aparecida Ribeiro1, Laize Peron Tófolo1, Latifa Abdennebi-Najar3, Didier Vieau4, Luiz Felipe Barella1, Paulo Cezar de Freitas Mathias1, Patrícia Cristina Lisboa2, Júlio Cezar de Oliveira5,6,7. 1. Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetic and Cell Biology, State University of Maringa/UEM, Block H67, Room 19, Colombo Avenue 5790, Maringá, PR, 87020-900, Brazil. 2. Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro, Rio De Janeiro, RJ, Brazil. 3. Institut Polytechnique LaSalle Beauvais, EGEAL-UP 2012.10.101, Beauvais Cedex, France. 4. Maternal Perinatal Undernutrition Team, Perinatal Environment and Growth Laboratory, Lille-North of France University, University of Sciences and Technologies of Lille, Villeneuve d'Ascq Cedex, France. 5. Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetic and Cell Biology, State University of Maringa/UEM, Block H67, Room 19, Colombo Avenue 5790, Maringá, PR, 87020-900, Brazil. biojborges@gmail.com. 6. Laboratory of Endocrine Physiology, Department of Physiological Sciences, Biology Institute, State University of Rio de Janeiro, Rio De Janeiro, RJ, Brazil. biojborges@gmail.com. 7. Health Sciences Institute, Federal University of Mato Grosso, Sinop, MT, Brazil. biojborges@gmail.com.
Abstract
PURPOSE: The long-term effects of the development of chronic metabolic diseases such as type 2 diabetes and obesity have been associated with nutritional insults in critical life stages. In this study, we evaluated the effect of a low-protein diet on metabolism in mid-adulthood male rats. METHODS: At 90 days of age, Wistar male rats were fed a low-protein diet (4.0 %, LP group) for 30 days, whereas control rats were fed a normal-protein diet (20.5 %, NP group) throughout their lifetimes. To allow for dietary rehabilitation, from 120 to 180 days of age, the LP rats were fed a normal-protein diet. Then, we measured body composition, fat stores, glucose-insulin homeostasis and pancreatic islet function. RESULTS: At 120 days of age, just after low-protein diet treatment, the LP rats displayed a strong lean phenotype, hypoinsulinemia, as assessed under fasting and glucose tolerance test conditions, as well as weak pancreatic islet insulinotropic response to glucose and acetylcholine (p < 0.01). At 180 days of age, after poor-protein diet rehabilitation, the LP rats displayed a slight lean phenotype (p < 0.05), which was associated with a high body weight gain (p < 0.001). Additionally, fat pad accumulation, glycemia and insulinemia, as well as the pancreatic islet insulinotropic response, were not significantly different between the LP and NP rats (p > 0.05). CONCLUSIONS: Taken together, the present data suggest that the effects of dietary restriction as a stressor in adulthood are reversible with dietary rehabilitation, indicating that adulthood is not a sensitive or critical time window for metabolic programming.
PURPOSE: The long-term effects of the development of chronic metabolic diseases such as type 2 diabetes and obesity have been associated with nutritional insults in critical life stages. In this study, we evaluated the effect of a low-protein diet on metabolism in mid-adulthood male rats. METHODS: At 90 days of age, Wistar male rats were fed a low-protein diet (4.0 %, LP group) for 30 days, whereas control rats were fed a normal-protein diet (20.5 %, NP group) throughout their lifetimes. To allow for dietary rehabilitation, from 120 to 180 days of age, the LPrats were fed a normal-protein diet. Then, we measured body composition, fat stores, glucose-insulin homeostasis and pancreatic islet function. RESULTS: At 120 days of age, just after low-protein diet treatment, the LPrats displayed a strong lean phenotype, hypoinsulinemia, as assessed under fasting and glucose tolerance test conditions, as well as weak pancreatic islet insulinotropic response to glucose and acetylcholine (p < 0.01). At 180 days of age, after poor-protein diet rehabilitation, the LPrats displayed a slight lean phenotype (p < 0.05), which was associated with a high body weight gain (p < 0.001). Additionally, fat pad accumulation, glycemia and insulinemia, as well as the pancreatic islet insulinotropic response, were not significantly different between the LP and NP rats (p > 0.05). CONCLUSIONS: Taken together, the present data suggest that the effects of dietary restriction as a stressor in adulthood are reversible with dietary rehabilitation, indicating that adulthood is not a sensitive or critical time window for metabolic programming.
Authors: J C de Oliveira; D X Scomparin; A E Andreazzi; R C S Branco; A G Martins; C Gravena; S Grassiolli; W Rinaldi; F B Barbosa; P C F Mathias Journal: J Neuroendocrinol Date: 2011-02 Impact factor: 3.627
Authors: A T S Fagundes; E G Moura; M C F Passos; E Oliveira; F P Toste; I T Bonomo; I H Trevenzoli; R M G Garcia; P C Lisboa Journal: Br J Nutr Date: 2007-05-25 Impact factor: 3.718
Authors: Douglas Lopes Almeida; Fernando Salgueiro Simões; Lucas Paulo Jacinto Saavedra; Ana Maria Praxedes Moraes; Camila Cristina Ianoni Matiusso; Ananda Malta; Kesia Palma-Rigo; Paulo Cesar de Freitas Mathias Journal: Endocrine Date: 2018-08-20 Impact factor: 3.633
Authors: Carlos A Ibáñez; Rafaela P Erthal; Fernanda M Ogo; Maria N C Peres; Henrique R Vieira; Camila Conejo; Laize P Tófolo; Flávio A Francisco; Sandra da Silva Silveira; Ananda Malta; Audrei Pavanello; Isabela P Martins; Paulo H O da Silva; Lucas Paulo Jacinto Saavedra; Gessica D Gonçalves; Veridiana M Moreira; Vander S Alves; Claudinéia C da Silva Franco; Carina Previate; Rodrigo M Gomes; Renan de Oliveira Venci; Francielle R S Dias; James A Armitage; Elena Zambrano; Paulo C F Mathias; Glaura S A Fernandes; Kesia Palma-Rigo Journal: Front Physiol Date: 2017-11-02 Impact factor: 4.566