| Literature DB >> 26123611 |
Lindsey O'Neal1, Alexandra J Obregón-Tito2, Raul Y Tito2, Andrew T Ozga2, Susan I Polo2, Cecil M Lewis2, Paul A Lawson3.
Abstract
A strictly anaerobic Gram-stain positive, spore-forming, rod-shaped bacterium designated NE08V(T), was isolated from a fecal sample of an individual residing in a remote Amazonian community in Peru. Phylogenetic analysis based on the 16S rRNA gene sequence showed the organism belonged to the genus Clostridium and is most closely related to Clostridium vulturis (97.4% sequence similarity) and was further characterized using biochemical and chemotaxonomic methods. The major cellular fatty acids were anteiso C13:0 and C16:0 with a genomic DNA G + C content of 31.6 mol%. Fermentation products during growth with PYG were acetate and butyrate. Based on phylogenetic, phenotypic and chemotaxonomic information, strain NE08V was identified as representing a novel species of the genus Clostridium, for which the name Clostridium amazonense sp. nov. is proposed. The type strain is NE08V(T) (DSM 23598(T) = CCUG 59712(T)).Entities:
Keywords: 16S rRNA phylogeny; Clostridium amazonense sp. nov.; Indigenous community; Taxonomy
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Year: 2015 PMID： 26123611 PMCID： PMC4600439 DOI： 10.1016/j.anaerobe.2015.06.005
Source DB: PubMed Journal: Anaerobe ISSN： 1075-9964 Impact factor: 3.331